A calcium-activated polyphosphoinositide phosphodiesterase in the plasma membrane of human and rabbit erythrocytes - PubMed (original) (raw)
A calcium-activated polyphosphoinositide phosphodiesterase in the plasma membrane of human and rabbit erythrocytes
D Allan et al. Biochim Biophys Acta. 1978.
Abstract
Haemoglobin-free human erythrocyte ghosts that were prepared in the presence of EDTA and were then exposed to Ca2+ showed a substantial loss of phosphatidylinositol phosphate and phosphatidylinositol diphosphate, measured either chemically or by loss of 32P from the lipids of prelabelled membranes. At the same time there was, as reported previously (Allan, D. and Michell, R.H., (1976) Biochim. Biophys. Acta 455, 824--830), and approximately equivalent rise in the diacylglycerol content of the membranes. Analysis of the 32P-labelled water-soluble material released during this process showed that the major products were inositol diphosphate and inositol triphosphate. No change was seen in the phosphatidylinositol or phosphatidate content of the membranes, and there was no Ca2+-activated loss of 32P from the phosphatidate of prelabelled membranes: this suggests that Ca2+ did not activate phosphoinositide phosphomonoesterases or phosphatidate phosphomonoesterase in human erythrocyte membranes. It is concluded that human erythrocyte membranes contain at their cytoplasmic surface a Ca2+-activated phosphodiesterase that is active against both phosphatidylinositol phosphate and phosphatidylinositol diphosphate. Rabbit erythrocytes also contained this enzyme, but in these cells there was also evidence for the presence of a Ca2+-activated phosphatidate phosphomonoesterase.
Similar articles
- The polyphosphoinositide phosphodiesterase of erythrocyte membranes.
Downes CP, Michell RH. Downes CP, et al. Biochem J. 1981 Jul 15;198(1):133-40. doi: 10.1042/bj1980133. Biochem J. 1981. PMID: 6275838 Free PMC article. - Phosphoinositide reorganization in human erythrocyte membrane upon cholesterol depletion.
M'Zali H, Giraud F. M'Zali H, et al. Biochem J. 1986 Feb 15;234(1):13-20. doi: 10.1042/bj2340013. Biochem J. 1986. PMID: 3010950 Free PMC article. - Ca2+-stimulated phospholipid phosphoesterase activities in rabbit erythrocyte membranes.
Quist E. Quist E. Arch Biochem Biophys. 1985 Jan;236(1):140-9. doi: 10.1016/0003-9861(85)90613-7. Arch Biochem Biophys. 1985. PMID: 2981504 - Polyphosphoinositide metabolism in excitable membranes. Review.
Hawthorne JN. Hawthorne JN. Biosci Rep. 1983 Oct;3(10):887-904. doi: 10.1007/BF01140658. Biosci Rep. 1983. PMID: 6317077 Review. No abstract available. - Metabolism of phosphatidate at the plasma membrane.
Allan D, Michell RH. Allan D, et al. Biochem Soc Trans. 1977;5(1):55-9. doi: 10.1042/bst0050055. Biochem Soc Trans. 1977. PMID: 196963 Review. No abstract available.
Cited by
- Control of the erythrocyte membrane shape: recovery from the effect of crenating agents.
Alhanaty E, Sheetz MP. Alhanaty E, et al. J Cell Biol. 1981 Dec;91(3 Pt 1):884-8. doi: 10.1083/jcb.91.3.884. J Cell Biol. 1981. PMID: 7328127 Free PMC article. - A 20 kDa erythrocyte membrane phosphoprotein.
Lee S, Cunningham EB, Swislocki NI. Lee S, et al. Mol Cell Biochem. 1991 Jul 24;106(1):75-85. doi: 10.1007/BF00231191. Mol Cell Biochem. 1991. PMID: 1656209 - The fatty acid composition of 1,2-diacylglycerol and polyphosphoinositides from human erythrocyte membranes.
Allan D, Cockcroft S. Allan D, et al. Biochem J. 1983 Aug 1;213(2):555-7. doi: 10.1042/bj2130555. Biochem J. 1983. PMID: 6311167 Free PMC article. - Ca2+-induced polyphosphoinositide breakdown due to phosphomonoesterase activity in chicken erythrocytes.
Raval PJ, Allan D. Raval PJ, et al. Biochem J. 1985 Oct 1;231(1):179-83. doi: 10.1042/bj2310179. Biochem J. 1985. PMID: 2998339 Free PMC article.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Miscellaneous