Expression and frameshifting but extremely inefficient proteolytic processing of the HIV-1 gag and pol gene products in stably transfected rodent cell lines - PubMed (original) (raw)
Expression and frameshifting but extremely inefficient proteolytic processing of the HIV-1 gag and pol gene products in stably transfected rodent cell lines
D Moosmayer et al. Virology. 1991 Jul.
Abstract
Expression, ribosomal frameshifting, and proteolytic processing of HIV-1 GAG and POL proteins were investigated in heterologous mammalian cells in order to elucidate the influence of the cellular background on these events. DNA fragments encoded by the gag and pol region were expressed in two rodent cell lines, LTK- and BHK. Both stably transfected cell lines continuously produce recombinant proteins which react with HIV-specific antisera. The GAG precursor and a 39-kDa proteolytic fragment thereof were the major recombinant proteins detected. Expression of the gag-pol region leads to the production of the GAG-POL precursor. Ribosomal frameshifting at the HIV-1 shifty sequence to a typical extent could be positively demonstrated by an enzyme assay. Despite the presence of the viral protease within the GAG-POL precursors, proteolytic processing of the HIV-derived polyproteins was extremely inefficient. The efficiency could not be enhanced by overexpression of the HIV-1 protease encoding region.
Similar articles
- Extensive regions of pol are required for efficient human immunodeficiency virus polyprotein processing and particle maturation.
Quillent C, Borman AM, Paulous S, Dauguet C, Clavel F. Quillent C, et al. Virology. 1996 May 1;219(1):29-36. doi: 10.1006/viro.1996.0219. Virology. 1996. PMID: 8623542 - Proteolytic processing of foamy virus Gag and Pol proteins.
Flügel RM, Pfrepper KI. Flügel RM, et al. Curr Top Microbiol Immunol. 2003;277:63-88. doi: 10.1007/978-3-642-55701-9_3. Curr Top Microbiol Immunol. 2003. PMID: 12908768 Review. - The packaging and maturation of the HIV-1 Pol proteins.
Hill M, Tachedjian G, Mak J. Hill M, et al. Curr HIV Res. 2005 Jan;3(1):73-85. doi: 10.2174/1570162052772942. Curr HIV Res. 2005. PMID: 15638725 Review.
Cited by
- Specific effects of ribosome-tethered molecular chaperones on programmed -1 ribosomal frameshifting.
Muldoon-Jacobs KL, Dinman JD. Muldoon-Jacobs KL, et al. Eukaryot Cell. 2006 Apr;5(4):762-70. doi: 10.1128/EC.5.4.762-770.2006. Eukaryot Cell. 2006. PMID: 16607023 Free PMC article. - Chimeric human immunodeficiency virus type 1 containing murine leukemia virus matrix assembles in murine cells.
Reed M, Mariani R, Sheppard L, Pekrun K, Landau NR, Soong NW. Reed M, et al. J Virol. 2002 Jan;76(1):436-43. doi: 10.1128/jvi.76.1.436-443.2002. J Virol. 2002. PMID: 11739711 Free PMC article. - A block to human immunodeficiency virus type 1 assembly in murine cells.
Mariani R, Rutter G, Harris ME, Hope TJ, Kräusslich HG, Landau NR. Mariani R, et al. J Virol. 2000 Apr;74(8):3859-70. doi: 10.1128/jvi.74.8.3859-3870.2000. J Virol. 2000. PMID: 10729160 Free PMC article. - Astrovirus ribosomal frameshifting in an infection-transfection transient expression system.
Lewis TL, Matsui SM. Lewis TL, et al. J Virol. 1996 May;70(5):2869-75. doi: 10.1128/JVI.70.5.2869-2875.1996. J Virol. 1996. PMID: 8627761 Free PMC article. - Translational frameshifting mediated by a viral sequence in plant cells.
Brault V, Miller WA. Brault V, et al. Proc Natl Acad Sci U S A. 1992 Mar 15;89(6):2262-6. doi: 10.1073/pnas.89.6.2262. Proc Natl Acad Sci U S A. 1992. PMID: 1549592 Free PMC article.
References
- Artelt P., Morelle C., Ausmeier M., Fitzek M., Hauser H. Vectors for efficient expression in mammalian fibroblast, myeloid and lymphoid cells via transfection or infection. Gene. 1988;68:213–219. - PubMed
- Barr P.J., Power M.D., Lee-Ng C.T., Gibson H.L., Luclw P.A. Expression of active human immunodeficiency virus reverse transcriptase in Saccharomyces cerevisiae. Biotechnology. 1987;5:486–489.
- Colbère-Garapin F., Horodniceau F., Khourilsky P., Garapin A.C. A new dominant hybrid selective marker for higher eukaryotic cells. J. Mol. Biol. 1981;150:1–13. - PubMed
- Dayton A.I., Terwilliger E.F., Potz J., Kowalski M., Sodroski I.G., Haseltine W.A. Cis-acting sequences responsive to the rev gene product of the human immunodeficiency virus. J. Acquired Immune Defic. Syndr. 1988;1:441–452. - PubMed
MeSH terms
Substances
LinkOut - more resources
Full Text Sources