Clinical relevance and diversity of two homologous genes encoding glycosyltransferases in Helicobacter pylori - PubMed (original) (raw)

Clinical relevance and diversity of two homologous genes encoding glycosyltransferases in Helicobacter pylori

Mónica Oleastro et al. J Clin Microbiol. 2010 Aug.

Abstract

Helicobacter pylori is known to be a major cause of peptic ulceration. The jhp0562 gene, encoding a glycosyltransferase involved in the synthesis of the lipopolysaccharide, was associated with peptic ulcer disease (PUD) in children. The beta-(1,3)-galactosyltransferase [beta-(1,3)GalT] gene (jhp0563), involved in Lewis (Le) antigen expression, is highly similar to jhp0562. The clinical significance and diversity of both genes were examined by PCR and sequencing of clinical strains (n = 117) isolated from children with PUD (n = 57) and nonulcer dyspepsia (NUD; n = 60). The prevalence of the jhp0562 gene was significantly higher in strains with a more-virulent profile (strains positive for the cag pathogenicity island [PAI], vacA sl allele, babA, homB, phase-variable gene oipA "on" [i.e., functional], and hopQ I allele). The distribution of genotypes according to clinical outcome showed that the presence of jhp0562 represented one of the greatest risks for the development of PUD. Moreover, the triple-positive genotype for the cag PAI, jhp0562, and homB provided the best discriminatory model for distinguishing PUD and NUD outcomes in children. Sequence and in vitro expression analyses of jhp0562 showed the presence of a complete open reading frame, while the beta-(1,3)GalT gene was shown to be a phase-variable gene. The regular presence of jhp0562 in strains with a truncated beta-(1,3)GalT gene suggests that jhp0562 may also be implicated in the regulation of Le antigen expression. Overall, the results of this study suggest that the jhp0562 gene is of great clinical relevance, being a useful comarker for severe H. pylori-related disease and contributing to host adaptation.

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Figures

FIG. 1.

FIG. 1.

Analysis of the association of Helicobacter pylori virulence factors between themselves and according to clinical outcome. Complete data (for the cag PAI, vacA s1 allele, babA, homB, oipA and sabA status, and hopQ and hopZ alleles) were available for 83 H. pylori strains isolated from children with peptic ulcer disease (n = 41) and nonulcer dyspepsia (n = 42). (A) Principal-component analysis plot. (B) Dendrogram constructed using the average aggregation method and Euclidian distance.

FIG. 2.

FIG. 2.

jhp0562 and β-(1,3)GalT gene profiles observed in Helicobacter pylori strains. The gene profiles were determined by PCR performed on 117 Helicobacter pylori strains and amplicons were separated on a 1.5% agarose gel. MW, molecular weight marker X174 RF DNA/HaeIII fragments (72 to 1,353 bp).

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