A mouse knockout library for secreted and transmembrane proteins - PubMed (original) (raw)

doi: 10.1038/nbt.1644. Epub 2010 Jun 20.

Li Li, Jerry Tang, Yun Li, Wei Yu Lin, Flavius Martin, Deanna Grant, Mark Solloway, Leon Parker, Weilan Ye, William Forrest, Nico Ghilardi, Tamas Oravecz, Kenneth A Platt, Dennis S Rice, Gwenn M Hansen, Alejandro Abuin, Derek E Eberhart, Paul Godowski, Kathleen H Holt, Andrew Peterson, Brian P Zambrowicz, Frederic J de Sauvage

Affiliations

• PMID: 20562862
• DOI: 10.1038/nbt.1644

A mouse knockout library for secreted and transmembrane proteins

Tracy Tang et al. Nat Biotechnol. 2010 Jul.

Abstract

Large collections of knockout organisms facilitate the elucidation of gene functions. Here we used retroviral insertion or homologous recombination to disrupt 472 genes encoding secreted and membrane proteins in mice, providing a resource for studying a large fraction of this important class of drug target. The knockout mice were subjected to a systematic phenotypic screen designed to uncover alterations in embryonic development, metabolism, the immune system, the nervous system and the cardiovascular system. The majority of knockout lines exhibited altered phenotypes in at least one of these therapeutic areas. To our knowledge, a comprehensive phenotypic assessment of a large number of mouse mutants generated by a gene-specific approach has not been described previously.

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Comment in

• Systematic phenotyping of mouse mutants.
  Wurst W, de Angelis MH. Wurst W, et al. Nat Biotechnol. 2010 Jul;28(7):684-5. doi: 10.1038/nbt0710-684. Nat Biotechnol. 2010. PMID: 20622838 No abstract available.

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