An 8-gene qRT-PCR-based gene expression score that has prognostic value in early breast cancer - PubMed (original) (raw)

doi: 10.1186/1471-2407-10-336.

Angelo Gámez-Pozo, Alvaro Pinto, David Hardisson, Rosario Madero, Rocío López, Belén San José, Pilar Zamora, Andrés Redondo, Jaime Feliu, Paloma Cejas, Manuel González Barón, Juan Angel Fresno Vara, Enrique Espinosa

Affiliations

An 8-gene qRT-PCR-based gene expression score that has prognostic value in early breast cancer

Iker Sánchez-Navarro et al. BMC Cancer. 2010.

Abstract

Background: Gene expression profiling may improve prognostic accuracy in patients with early breast cancer. Our objective was to demonstrate that it is possible to develop a simple molecular signature to predict distant relapse.

Methods: We included 153 patients with stage I-II hormonal receptor-positive breast cancer. RNA was isolated from formalin-fixed paraffin-embedded samples and qRT-PCR amplification of 83 genes was performed with gene expression assays. The genes we analyzed were those included in the 70-Gene Signature, the Recurrence Score and the Two-Gene Index. The association among gene expression, clinical variables and distant metastasis-free survival was analyzed using Cox regression models.

Results: An 8-gene prognostic score was defined. Distant metastasis-free survival at 5 years was 97% for patients defined as low-risk by the prognostic score versus 60% for patients defined as high-risk. The 8-gene score remained a significant factor in multivariate analysis and its performance was similar to that of two validated gene profiles: the 70-Gene Signature and the Recurrence Score. The validity of the signature was verified in independent cohorts obtained from the GEO database.

Conclusions: This study identifies a simple gene expression score that complements histopathological prognostic factors in breast cancer, and can be determined in paraffin-embedded samples.

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Figures

Figure 1

Figure 1

Kaplan-Meier curves showing distant metastasis-free survival (DMFS) for low-risk and high-risk patient groups (as defined by the 8-gene Score). A: all 153 patients included in this study B: patients with positive lymph nodes C: patients with negative lymph nodes D: patients who did not receive chemotherapy

Figure 2

Figure 2

Distant metastasis-free survival (DMFS) rates as a continuous function of the 8-gene Score. The red line indicates the cutoff point. Dashed lines indicate the 95% confidence interval.

Figure 3

Figure 3

Kaplan-Meier curves showing distant metastasis-free survival (DMFS) for patients included in four databases available online.

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