Anti-Tribbles homolog 2 autoantibodies in Japanese patients with narcolepsy - PubMed (original) (raw)

Anti-Tribbles homolog 2 autoantibodies in Japanese patients with narcolepsy

Hiromi Toyoda et al. Sleep. 2010 Jul.

Abstract

Study objectives: Narcolepsy is a sleep disorder characterized by excessive daytime sleepiness and cataplexy. The association with human leukocyte antigen (HLA)-DQB1*0602 and T-cell receptor alpha locus suggests that autoimmunity plays a role in narcolepsy. A recent study reported an increased prevalence of autoantibodies against Tribbles homolog 2 (TRIB2) in patients with narcolepsy. To replicate this finding, we examined anti-TRIB2 autoantibodies in Japanese patients with narcolepsy.

Design: We examined anti-TRIB2 autoantibodies against a full-length [35S]-labeled TRIB2 antigen in Japanese patients with narcolepsy-cataplexy (n = 88), narcolepsy without cataplexy (n = 18), and idiopathic hypersomnia with long sleep time (n = 11). The results were compared to Japanese healthy controls (n = 87). Thirty-seven healthy control subjects were positive for HLA-DRB1*1501-DQB1*0602. We also examined autoantibodies against another Tribbles homolog, TRIB3, as an experimental control.

Measurements and results: Autoantibodies against TRIB2 were found in 26.1% of patients with narcolepsy-cataplexy, a significantly higher prevalence than the 2.3% in healthy controls. We found that anti-TRIB3 autoantibodies were rare in patients with narcolepsy and showed no association with anti-TRIB2 indices. No significant correlation was found between anti-TRIB2 positivity and clinical information.

Conclusions: We confirmed the higher prevalence and specificity of anti-TRIB2 autoantibodies in Japanese patients with narcolepsy-cataplexy. This suggests a subgroup within narcolepsy-cataplexy might be affected by an anti-TRIB2 autoantibody-mediated autoimmune mechanism.

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Figure 1

Figure 1

Autoantibody index of human TRIB2. Indices of autoantibodies in patients with narcolepsy-cataplexy, narcolepsy without cataplexy, and idiopathic hypersomnia with long sleep time, and healthy controls are represented. Each dot corresponds to data from one subject. The dotted line indicates the mean + 2SD levels in healthy controls. The indices with subjects above this line were considered to be positive. NA, narcolepsy with cataplexy; NAwoCA, narcolepsy without cataplexy; IHS, idiopathic hypersomnia with long sleep time; HC, healthy control.

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