Three-dimensional structure of the LDL receptor-binding domain of human apolipoprotein E - PubMed (original) (raw)

. 1991 Jun 28;252(5014):1817-22.

doi: 10.1126/science.2063194.

Affiliations

• PMID: 2063194
• DOI: 10.1126/science.2063194

Three-dimensional structure of the LDL receptor-binding domain of human apolipoprotein E

C Wilson et al. Science. 1991.

Abstract

Human apolipoprotein E, a blood plasma protein, mediates the transport and uptake of cholesterol and lipid by way of its high affinity interaction with different cellular receptors, including the low-density lipoprotein (LDL) receptor. The three-dimensional structure of the LDL receptor-binding domain of apoE has been determined at 2.5 angstrom resolution by x-ray crystallography. The protein forms an unusually elongated (65 angstroms) four-helix bundle, with the helices apparently stabilized by a tightly packed hydrophobic core that includes leucine zipper-type interactions and by numerous salt bridges on the mostly charged surface. Basic amino acids important for LDL receptor binding are clustered into a surface patch on one long helix. This structure provides the basis for understanding the behavior of naturally occurring mutants that can lead to atherosclerosis.

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