Metabolomic profiles indicate distinct physiological pathways affected by two loci with major divergent effect on Bos taurus growth and lipid deposition - PubMed (original) (raw)

Metabolomic profiles indicate distinct physiological pathways affected by two loci with major divergent effect on Bos taurus growth and lipid deposition

Rosemarie Weikard et al. Physiol Genomics. 2010 Oct.

Abstract

Identifying trait-associated genetic variation offers new prospects to reveal novel physiological pathways modulating complex traits. Taking advantage of a unique animal model, we identified the I442M mutation in the non-SMC condensin I complex, subunit G (NCAPG) gene and the Q204X mutation in the growth differentiation factor 8 (GDF8) gene as substantial modulators of pre- and/or postnatal growth in cattle. In a combined metabolomic and genotype association approach, which is the first respective study in livestock, we surveyed the specific physiological background of the effects of both loci on body-mass gain and lipid deposition. Our data provided confirming evidence from two historically and geographically distant cattle populations that the onset of puberty is the key interval of divergent growth. The locus-specific metabolic patterns obtained from monitoring 201 plasma metabolites at puberty mirror the particular NCAPG I442M and GDF8 Q204X effects and represent biosignatures of divergent physiological pathways potentially modulating effects on proportional and disproportional growth, respectively. While the NCAPG I442M mutation affected the arginine metabolism, the 204X allele in the GDF8 gene predominantly raised the carnitine level and had concordant effects on glycerophosphatidylcholines and sphingomyelins. Our study provides a conclusive link between the well-described growth-regulating functions of arginine metabolism and the previously unknown specific physiological role of the NCAPG protein in mammalian metabolism. Owing to the confirmed effect of the NCAPG/LCORL locus on human height in genome-wide association studies, the results obtained for bovine NCAPG might add valuable, comparative information on the physiological background of genetically determined divergent mammalian growth.

PubMed Disclaimer

Cited by

Patterns of enrichment and acceleration in evolutionary rates of promoters suggest a role of regulatory regions in cetacean gigantism.
Silva FA, Picorelli ACR, Veiga GS, Nery MF. Silva FA, et al. BMC Ecol Evol. 2023 Oct 24;23(1):62. doi: 10.1186/s12862-023-02171-5. BMC Ecol Evol. 2023. PMID: 37872505 Free PMC article.
The molecular evolution of genes previously associated with large sizes reveals possible pathways to cetacean gigantism.
Silva FA, Souza ÉMS, Ramos E, Freitas L, Nery MF. Silva FA, et al. Sci Rep. 2023 Jan 19;13(1):67. doi: 10.1038/s41598-022-24529-3. Sci Rep. 2023. PMID: 36658131 Free PMC article.
Exploring and Identifying Candidate Genes and Genomic Regions Related to Economically Important Traits in Hanwoo Cattle.
Naserkheil M, Manzari Z, Dang CG, Lee SS, Park MN. Naserkheil M, et al. Curr Issues Mol Biol. 2022 Dec 4;44(12):6075-6092. doi: 10.3390/cimb44120414. Curr Issues Mol Biol. 2022. PMID: 36547075 Free PMC article. Review.
Genome-wide association analysis of milk production, somatic cell score, and body conformation traits in Holstein cows.
Wang P, Li X, Zhu Y, Wei J, Zhang C, Kong Q, Nie X, Zhang Q, Wang Z. Wang P, et al. Front Vet Sci. 2022 Oct 4;9:932034. doi: 10.3389/fvets.2022.932034. eCollection 2022. Front Vet Sci. 2022. PMID: 36268046 Free PMC article.
Genome-wide Association Study for Carcass Primal Cut Yields Using Single-step Bayesian Approach in Hanwoo Cattle.
Naserkheil M, Mehrban H, Lee D, Park MN. Naserkheil M, et al. Front Genet. 2021 Nov 26;12:752424. doi: 10.3389/fgene.2021.752424. eCollection 2021. Front Genet. 2021. PMID: 34899840 Free PMC article.

Metabolomic profiles indicate distinct physiological pathways affected by two loci with major divergent effect on Bos taurus growth and lipid deposition - PubMed (original) (raw)