Lutein improves antioxidant defense in vivo and protects against DNA damage and chromosome instability induced by cisplatin - PubMed (original) (raw)
. 2010 Oct;84(10):811-22.
doi: 10.1007/s00204-010-0576-y. Epub 2010 Aug 4.
Affiliations
- PMID: 20683581
- DOI: 10.1007/s00204-010-0576-y
Lutein improves antioxidant defense in vivo and protects against DNA damage and chromosome instability induced by cisplatin
Juliana Mara Serpeloni et al. Arch Toxicol. 2010 Oct.
Abstract
Lutein (LT) is the second most prevalent carotenoid in human serum, and it is abundantly present in dark, leafy green vegetables. The objectives of this study were to evaluate the genotoxicity and mutagenicity of LT, and its protective effects in vivo against DNA damage and chromosome instability induced by cisplatin (cDDP). For this purpose, we used the comet assay and micronucleus (MN) test, and we evaluated the antioxidant effects of LT by determination of enzymatic (catalase-CAT) and non-enzymatic (reduced glutathione-GSH) activity. Mice were divided into six groups: cDDP, mineral oil (OM), LT groups and LT + cDDP groups. To perform the MN test on peripheral blood (PB) cells, blood samples were collected before the first treatment (T0), and 36 h (T1) and 14 days (T2) after the first treatment. To perform the comet assay, blood samples were collected 4 h after the first and the last treatment. Oxidative capacity was analyzed in total blood that was collected 24 h after the last treatment, when bone marrow (BM) sample was also collected for the MN test. No genotoxic or mutagenic effects of LT were observed for the doses evaluated. We did find that this carotenoid was able to reduce the formation of crosslinks and chromosome instability induced by cDDP. No differences were observed in CAT levels, and LT treatment increased GSH levels compared with a negative control group, reinforcing the role of this carotenoid as an antioxidant.
Similar articles
- Effects of lutein and chlorophyll b on GSH depletion and DNA damage induced by cisplatin in vivo.
Serpeloni JM, Almeida MR, Mercadante AZ, Bianchi ML, Antunes LM. Serpeloni JM, et al. Hum Exp Toxicol. 2013 Aug;32(8):828-36. doi: 10.1177/0960327112468911. Hum Exp Toxicol. 2013. PMID: 23821640 - An evaluation, using the comet assay and the micronucleus test, of the antigenotoxic effects of chlorophyll b in mice.
Serpeloni JM, Grotto D, Aissa AF, Mercadante AZ, Bianchi Mde L, Antunes LM. Serpeloni JM, et al. Mutat Res. 2011 Oct 9;725(1-2):50-6. doi: 10.1016/j.mrgentox.2011.06.009. Epub 2011 Jul 6. Mutat Res. 2011. PMID: 21763449 - Dietary carotenoid lutein protects against DNA damage and alterations of the redox status induced by cisplatin in human derived HepG2 cells.
Serpeloni JM, Barcelos GR, Friedmann Angeli JP, Mercadante AZ, Lourdes Pires Bianchi M, Antunes LM. Serpeloni JM, et al. Toxicol In Vitro. 2012 Mar;26(2):288-94. doi: 10.1016/j.tiv.2011.11.011. Epub 2011 Nov 26. Toxicol In Vitro. 2012. PMID: 22138568 - Genotoxicity of environmental agents assessed by the alkaline comet assay.
Møller P. Møller P. Basic Clin Pharmacol Toxicol. 2005;96 Suppl 1:1-42. Basic Clin Pharmacol Toxicol. 2005. PMID: 15859009 Review. - Methods for the detection of antioxidants which prevent age related diseases: a critical review with particular emphasis on human intervention studies.
Hoelzl C, Bichler J, Ferk F, Simic T, Nersesyan A, Elbling L, Ehrlich V, Chakraborty A, Knasmüller S. Hoelzl C, et al. J Physiol Pharmacol. 2005 Mar;56 Suppl 2:49-64. J Physiol Pharmacol. 2005. PMID: 16077190 Review.
Cited by
- Healthy Diets from Sustainable Food Systems: Calculating the WISH Scores for Women in Rural East Africa.
Keding GB, Sarfo J, Pawelzik E. Keding GB, et al. Nutrients. 2023 Jun 9;15(12):2699. doi: 10.3390/nu15122699. Nutrients. 2023. PMID: 37375603 Free PMC article. - Genome-Protecting Compounds as Potential Geroprotectors.
Proshkina E, Shaposhnikov M, Moskalev A. Proshkina E, et al. Int J Mol Sci. 2020 Jun 24;21(12):4484. doi: 10.3390/ijms21124484. Int J Mol Sci. 2020. PMID: 32599754 Free PMC article. Review. - Heat treatment and protective potentials of luteolin-7-O-glucoside against cisplatin genotoxic and cytotoxic effects.
Maatouk M, Abed B, Bouhlel I, Krifa M, Khlifi R, Ioannou I, Ghedira K, Ghedira LC. Maatouk M, et al. Environ Sci Pollut Res Int. 2020 Apr;27(12):13417-13427. doi: 10.1007/s11356-020-07900-7. Epub 2020 Feb 5. Environ Sci Pollut Res Int. 2020. PMID: 32026362 - Cereus jamacaru D.C. Hydroalcoholic Extract Promotes Anti-Cytotoxic and Antitumor Activity.
Vencioneck Dutra JC, Moisés Ferreira J, Costalonga Pereira PR, Ben-Hur de Oliveira J, Vitorino Gervásio S, Bernardes Xavier M, Mantovanelli da Mota M, Luz ACD, Rodrigues Pretti I, Seibert França H, Masrouah Jamal C, Pimentel Batitucci MDC. Vencioneck Dutra JC, et al. Pharmaceuticals (Basel). 2018 Nov 23;11(4):130. doi: 10.3390/ph11040130. Pharmaceuticals (Basel). 2018. PMID: 30477180 Free PMC article. - Burn-Induced Multiple Organ Injury and Protective Effect of Lutein in Rats.
AbuBakr HO, Aljuaydi SH, Abou-Zeid SM, El-Bahrawy A. AbuBakr HO, et al. Inflammation. 2018 Jun;41(3):760-772. doi: 10.1007/s10753-018-0730-x. Inflammation. 2018. PMID: 29417308
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Miscellaneous