Peptidoglycan recognition proteins protect mice from experimental colitis by promoting normal gut flora and preventing induction of interferon-gamma - PubMed (original) (raw)

Figure 3. Higher expression of IFN-inducible genes in the colon in PGRP-deficient than in WT mice following DSS treatment

A. Top 75 genes expressed higher in the colon of PGRP-deficient than WT mice after 2 days of oral treatment with 5% DSS were identified by whole genome expression array as mostly IFN-inducible genes. Their expression is shown here measured by qRT-PCR. The results are the ratio of the amount of mRNA in DSS-treated to untreated mice. The gene symbols are (IFN-inducible genes are in bold face): A1-12: Iigp1, Tgtp, Indo1, Ifit2, H28, Ifi44, Igtp, Upp1, Cxcl10, Gbp1, Cxcl9, Mpa2l; B1-12: Ifi47, Gbp2, Usp18, Slfn4, Gbp5, Gbp4, Zbp1, Trim30, Oas3, Irgm1, Apol9b, Gbp6; C1-12: Oas2, Ifit3, Tap1, Oasl2, Stat1, Herc5, Ifit1, Tnfsf10, Mx2, Ccl8, Ly6a, Psmb8; D1-12: Rsad2, Phf11, Cmpk2, Cxcl11, Ms4a1, Nlrc5, Ddx60, Ly6e, Nos2, Isg15, Mx1, Slfn2; E1-12: Psmb9, Stat2, Reg3b, Gzmb, Insig1, Klhl6, Rtp4, Ube2l6, Xdh, Cd274, Cd72, Rnf213; F1-12: Dhx58, Ly6c1, Fcgr4, Trim15, Art2a, Cd19, Batf2, Parp9, Pbef1 , Parp14, Ifi27, Cxcr5; G1-3: Mlkl, Lgals9, Cd74. Gene descriptions and detailed results are shown in Table S3. B and C. IFN-γ and IFN-inducible chemokines are preferentially induced by DSS treatment in the colon in PGRP-deficient but not in WT mice. B. Top 6 inflammatory genes expressed higher in the colon of PGRP-deficient than WT mice after 2 days of oral treatment with 5% DSS out of a panel of 84 cytokines, chemokines, and other marker genes for various immune cell types were identified by qRT-PCR as IFN-γ and IFN-inducible genes (CXCL-9, CXCL-10, CXCL-11, CCL-8, and iNOS-2), which are characteristic of an NK cell profile. The results are the ratio of the amount of mRNA in DSS-treated to untreated mice. Detailed results for all 84 genes are shown in Table S4. C. Kinetics of induction of IFN-γ and IFN-inducible chemokines in the colon in DSS-treated Pglyrp3−/− and WT mice; means of 3 assays on 5 mice/group ± SEM.