Myosin functional domains encoded by alternative exons are expressed in specific thoracic muscles of Drosophila - PubMed (original) (raw)
Myosin functional domains encoded by alternative exons are expressed in specific thoracic muscles of Drosophila
G A Hastings et al. J Cell Biol. 1991 Jul.
Abstract
The Drosophila 36B muscle myosin heavy chain (MHC) gene has five sets of alternatively spliced exons that encode functionally important domains of the MHC protein and provide a combinatorial potential for expression of as many as 480 MHC isoforms. In this study, in situ hybridization analysis has been used to examine the complexity and muscle specificity of MHC isoform expression in the fibrillar indirect flight muscle (IFM), the tubular direct flight muscles (DFM) and tubular tergal depressor of the trochanter muscle (TDT), and the visceral esophageal muscle in the adult thorax. Our results show that alternative splicing of the MHC gene transcripts is precisely regulated in these thoracic muscles, which express three MHC isoforms. Individual thoracic muscles each express transcripts of only one isoform, as detectable by in situ hybridization. An apparently novel fourth MHC isoform, with sequence homology to the rod but not to the head domain of the 36B MHC, is expressed in two direct flight muscles. These findings form a basis for transgenic experiments designed to analyze the muscle-specific functions of MHC domains encoded by alternative exons.
Similar articles
- Regulated functional alternative splicing in Drosophila.
Venables JP, Tazi J, Juge F. Venables JP, et al. Nucleic Acids Res. 2012 Jan;40(1):1-10. doi: 10.1093/nar/gkr648. Epub 2011 Sep 8. Nucleic Acids Res. 2012. PMID: 21908400 Free PMC article. Review. - Functional domains of the Drosophila melanogaster muscle myosin heavy-chain gene are encoded by alternatively spliced exons.
George EL, Ober MB, Emerson CP Jr. George EL, et al. Mol Cell Biol. 1989 Jul;9(7):2957-74. doi: 10.1128/mcb.9.7.2957-2974.1989. Mol Cell Biol. 1989. PMID: 2506434 Free PMC article. - An alternative domain near the nucleotide-binding site of Drosophila muscle myosin affects ATPase kinetics.
Miller BM, Zhang S, Suggs JA, Swank DM, Littlefield KP, Knowles AF, Bernstein SI. Miller BM, et al. J Mol Biol. 2005 Oct 14;353(1):14-25. doi: 10.1016/j.jmb.2005.08.012. J Mol Biol. 2005. PMID: 16154586 - Multiple mechanisms regulate muscle fiber diversity.
Gunning P, Hardeman E. Gunning P, et al. FASEB J. 1991 Dec;5(15):3064-70. doi: 10.1096/fasebj.5.15.1835946. FASEB J. 1991. PMID: 1835946 Review.
Cited by
- Genetic Control of Muscle Diversification and Homeostasis: Insights from Drosophila.
Poovathumkadavil P, Jagla K. Poovathumkadavil P, et al. Cells. 2020 Jun 25;9(6):1543. doi: 10.3390/cells9061543. Cells. 2020. PMID: 32630420 Free PMC article. Review. - Transcriptomic and Functional Analyses of Phenotypic Plasticity in a Higher Termite, Macrotermes barneyi Light.
Sun P, Li G, Jian J, Liu L, Chen J, Yu S, Xu H, Lei C, Zhou X, Huang Q. Sun P, et al. Front Genet. 2019 Oct 4;10:964. doi: 10.3389/fgene.2019.00964. eCollection 2019. Front Genet. 2019. PMID: 31681415 Free PMC article. - Alternative relay and converter domains tune native muscle myosin isoform function in Drosophila.
Kronert WA, Melkani GC, Melkani A, Bernstein SI. Kronert WA, et al. J Mol Biol. 2012 Mar 2;416(4):543-57. doi: 10.1016/j.jmb.2011.12.044. Epub 2011 Dec 28. J Mol Biol. 2012. PMID: 22226837 Free PMC article. - Regulated functional alternative splicing in Drosophila.
Venables JP, Tazi J, Juge F. Venables JP, et al. Nucleic Acids Res. 2012 Jan;40(1):1-10. doi: 10.1093/nar/gkr648. Epub 2011 Sep 8. Nucleic Acids Res. 2012. PMID: 21908400 Free PMC article. Review. - Mutating the converter-relay interface of Drosophila myosin perturbs ATPase activity, actin motility, myofibril stability and flight ability.
Kronert WA, Melkani GC, Melkani A, Bernstein SI. Kronert WA, et al. J Mol Biol. 2010 May 21;398(5):625-32. doi: 10.1016/j.jmb.2010.03.049. Epub 2010 Apr 1. J Mol Biol. 2010. PMID: 20362584 Free PMC article.
References
- Annu Rev Biochem. 1987;56:695-726 - PubMed
- J Muscle Res Cell Motil. 1990 Jun;11(3):203-15 - PubMed
- Cell. 1987 Oct 23;51(2):221-8 - PubMed
- Annu Rev Cell Biol. 1987;3:379-421 - PubMed
- Mol Cell Biol. 1988 Mar;8(3):1352-60 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Molecular Biology Databases
Research Materials