Changes in the expression of MMP-3, MMP-9, TIMP-1 and aggrecan in the condylar cartilage of rats induced by experimentally created disordered occlusion - PubMed (original) (raw)

Changes in the expression of MMP-3, MMP-9, TIMP-1 and aggrecan in the condylar cartilage of rats induced by experimentally created disordered occlusion

Guo-Wei Wang et al. Arch Oral Biol. 2010 Nov.

Abstract

Objective: To investigate the effects of experimentally created disordered occlusion on the mandibular condylar cartilage in terms of histological morphology and expression of MMP-3, MMP-9, TIMP-1 and aggrecan.

Materials and methods: Eighty 8-week-old Sprague-Dawley rats were randomly divided into two experimental (Exp) and two control (Con) groups, with equal sex and number distribution as subgroups. In the Exp group, the disordered occlusion was created by orthodontically moving the first and third molars 0.8mm away. Hematoxylin-eosin and immunohistochemical staining were performed on the mandibular condyles at the end of the 8th or 12th week. Gene expression was analysed by real-time PCR.

Results: Osteoarthritis-like lesions, typically seen as a cell-free area, were detected in the Exp group, predominantly in females. In the cell-free area, the immunopositive expression of MMP-3, MMP-9, TIMP-1 and aggrecan were absent. Hyper-proliferation changes, typically seen as conjunctive invaginations of chondrocytes, were also observed where immunopositive expression of the tested materials was strong. There were sex and time point related differences in gene expression. In the 8-week subgroup, the expression of MMP-3 decreased, while aggrecan increased in males; however, both MMP-9 and TIMP increased in the female group (P<0.05). In the 12-week subgroup, the expression of MMP-3 increased, while TIMP, MMP-9 (male only) and aggrecan (female only) decreased (P<0.05).

Conclusions: The present results indicate that the experimentally created disordered occlusion led to osteoarthritis-like lesions accompanied by changes in the expression of MMP-3, MMP-9, TIMP-1 and aggrecan in mandibular condyle cartilage with gender differences.

PubMed Disclaimer

Cited by

Occlusal effects on longitudinal bone alterations of the temporomandibular joint.
Zhang J, Jiao K, Zhang M, Zhou T, Liu XD, Yu SB, Lu L, Jing L, Yang T, Zhang Y, Chen D, Wang MQ. Zhang J, et al. J Dent Res. 2013 Mar;92(3):253-9. doi: 10.1177/0022034512473482. Epub 2013 Jan 22. J Dent Res. 2013. PMID: 23340211 Free PMC article.
The identification of CD163 expressing phagocytic chondrocytes in joint cartilage and its novel scavenger role in cartilage degradation.
Jiao K, Zhang J, Zhang M, Wei Y, Wu Y, Qiu ZY, He J, Cao Y, Hu J, Zhu H, Niu LN, Cao X, Yang K, Wang MQ. Jiao K, et al. PLoS One. 2013;8(1):e53312. doi: 10.1371/journal.pone.0053312. Epub 2013 Jan 11. PLoS One. 2013. PMID: 23326413 Free PMC article.
Animal Models of Temporomandibular Disorder.
Xiang T, Tao ZY, Liao LF, Wang S, Cao DY. Xiang T, et al. J Pain Res. 2021 May 26;14:1415-1430. doi: 10.2147/JPR.S303536. eCollection 2021. J Pain Res. 2021. PMID: 34079358 Free PMC article. Review.
TNF Accelerates Death of Mandibular Condyle Chondrocytes in Rats with Biomechanical Stimulation-Induced Temporomandibular Joint Disease.
Yang H, Zhang M, Wang X, Zhang H, Zhang J, Jing L, Liao L, Wang M. Yang H, et al. PLoS One. 2015 Nov 3;10(11):e0141774. doi: 10.1371/journal.pone.0141774. eCollection 2015. PLoS One. 2015. PMID: 26529096 Free PMC article.
What is the effect of nicotinic acetylcholine receptor stimulation on osteoarthritis in a rodent animal model?
Bock K, Plaass C, Coger V, Peck CT, Reimers K, Stukenborg-Colsman C, Claassen L. Bock K, et al. SAGE Open Med. 2016 Mar 9;4:2050312116637529. doi: 10.1177/2050312116637529. eCollection 2016. SAGE Open Med. 2016. PMID: 27026802 Free PMC article.

Changes in the expression of MMP-3, MMP-9, TIMP-1 and aggrecan in the condylar cartilage of rats induced by experimentally created disordered occlusion - PubMed (original) (raw)