Use of fluorescent in situ hybridization to detect chromosomal rearrangements in somatic cell hybrids - PubMed (original) (raw)
Use of fluorescent in situ hybridization to detect chromosomal rearrangements in somatic cell hybrids
A J Giaccia et al. Genes Chromosomes Cancer. 1990 Sep.
Abstract
In situ hybridization of hamster/human hybrids with biotinylated human genomic DNA has revealed that human chromosomal DNA can integrate into the hamster genome and is not always cytologically detectable. This finding helps to explain why discordancy can arise in gene mapping by failing to recognize small pieces of foreign DNA in the rodent genome. Fluorescent in situ hybridization allows one to locate these fragments in rodent chromosomes visually and possibly to identify their chromosome of origin.
Publication types
MeSH terms
LinkOut - more resources
Full Text Sources