RNA polymerase I contains a TFIIF-related DNA-binding subcomplex - PubMed (original) (raw)
RNA polymerase I contains a TFIIF-related DNA-binding subcomplex
Sebastian R Geiger et al. Mol Cell. 2010.
Free article
Abstract
The eukaryotic RNA polymerases Pol I, II, and III use different promoters to transcribe different classes of genes. Promoter usage relies on initiation factors, including TFIIF and TFIIE, in the case of Pol II. Here, we show that the Pol I-specific subunits A49 and A34.5 form a subcomplex that binds DNA and is related to TFIIF and TFIIE. The N-terminal regions of A49 and A34.5 form a dimerization module that stimulates polymerase-intrinsic RNA cleavage and has a fold that resembles the TFIIF core. The C-terminal region of A49 forms a "tandem winged helix" (tWH) domain that binds DNA with a preference for the upstream promoter nontemplate strand and is predicted in TFIIE. Similar domains are predicted in Pol III-specific subunits. Thus, Pol I/III subunits that have no counterparts in Pol II are evolutionarily related to Pol II initiation factors and may have evolved to mediate promoter specificity and transcription processivity.
Copyright (c) 2010 Elsevier Inc. All rights reserved.
Similar articles
- Functional architecture of RNA polymerase I.
Kuhn CD, Geiger SR, Baumli S, Gartmann M, Gerber J, Jennebach S, Mielke T, Tschochner H, Beckmann R, Cramer P. Kuhn CD, et al. Cell. 2007 Dec 28;131(7):1260-72. doi: 10.1016/j.cell.2007.10.051. Cell. 2007. PMID: 18160037 - Dynamics of the RNA polymerase I TFIIF/TFIIE-like subcomplex: a mini-review.
Knutson BA, McNamar R, Rothblum LI. Knutson BA, et al. Biochem Soc Trans. 2020 Oct 30;48(5):1917-1927. doi: 10.1042/BST20190848. Biochem Soc Trans. 2020. PMID: 32915199 Free PMC article. Review. - Position of the general transcription factor TFIIF within the RNA polymerase II transcription preinitiation complex.
Eichner J, Chen HT, Warfield L, Hahn S. Eichner J, et al. EMBO J. 2010 Feb 17;29(4):706-16. doi: 10.1038/emboj.2009.386. Epub 2009 Dec 24. EMBO J. 2010. PMID: 20033062 Free PMC article. - Functions of the TFIIE-Related Tandem Winged-Helix Domain of Rpc34 in RNA Polymerase III Initiation and Elongation.
Wei YY, Chen HT. Wei YY, et al. Mol Cell Biol. 2018 Jan 29;38(4):e00105-17. doi: 10.1128/MCB.00105-17. Print 2018 Feb 15. Mol Cell Biol. 2018. PMID: 29180511 Free PMC article. - Specialization versus conservation: How Pol I and Pol III use the conserved architecture of the pre-initiation complex for specialized transcription.
Hoffmann NA, Sadian Y, Tafur L, Kosinski J, Müller CW. Hoffmann NA, et al. Transcription. 2016 Aug 7;7(4):127-32. doi: 10.1080/21541264.2016.1203628. Epub 2016 Jun 21. Transcription. 2016. PMID: 27327079 Free PMC article. Review.
Cited by
- Structural and functional analyses of the interaction of archaeal RNA polymerase with DNA.
Wojtas MN, Mogni M, Millet O, Bell SD, Abrescia NG. Wojtas MN, et al. Nucleic Acids Res. 2012 Oct;40(19):9941-52. doi: 10.1093/nar/gks692. Epub 2012 Jul 30. Nucleic Acids Res. 2012. PMID: 22848102 Free PMC article. - The diverse and expanding role of mass spectrometry in structural and molecular biology.
Lössl P, van de Waterbeemd M, Heck AJ. Lössl P, et al. EMBO J. 2016 Dec 15;35(24):2634-2657. doi: 10.15252/embj.201694818. Epub 2016 Oct 26. EMBO J. 2016. PMID: 27797822 Free PMC article. Review. - The initiation factor TFE and the elongation factor Spt4/5 compete for the RNAP clamp during transcription initiation and elongation.
Grohmann D, Nagy J, Chakraborty A, Klose D, Fielden D, Ebright RH, Michaelis J, Werner F. Grohmann D, et al. Mol Cell. 2011 Jul 22;43(2):263-74. doi: 10.1016/j.molcel.2011.05.030. Mol Cell. 2011. PMID: 21777815 Free PMC article. - Basic mechanisms in RNA polymerase I transcription of the ribosomal RNA genes.
Goodfellow SJ, Zomerdijk JC. Goodfellow SJ, et al. Subcell Biochem. 2013;61:211-36. doi: 10.1007/978-94-007-4525-4_10. Subcell Biochem. 2013. PMID: 23150253 Free PMC article. Review. - DNA-dependent RNA polymerases in plants.
Yang DL, Huang K, Deng D, Zeng Y, Wang Z, Zhang Y. Yang DL, et al. Plant Cell. 2023 Sep 27;35(10):3641-3661. doi: 10.1093/plcell/koad195. Plant Cell. 2023. PMID: 37453082 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases