The matrix protein hnRNP U is required for chromosomal localization of Xist RNA - PubMed (original) (raw)
The matrix protein hnRNP U is required for chromosomal localization of Xist RNA
Yuko Hasegawa et al. Dev Cell. 2010.
Free article
Abstract
In XX female mammals, one of the two X chromosomes is epigenetically inactivated to equalize gene expression with XY males. The formation of the inactive X chromosome (Xi) is regulated by an X-linked long noncoding RNA Xist, which accumulates on the entire length of the chromosome in cis and induces heterochromatin formation. However, the mechanism by which Xist RNA "paints" the Xi has long remained elusive. Here, we show that a matrix protein hnRNP U/SP120/SAF-A is required for the accumulation of Xist RNA on the Xi. Xist RNA and hnRNP U interact and upon depletion of hnRNP U, Xist RNA is detached from the Xi and diffusely localized into the nucleoplasm. In addition, ES cells lacking hnRNP U expression fail to form the Xi. We propose that the association with matrix proteins is an essential step in the epigenetic regulation of gene expression by Xist RNA.
Copyright © 2010 Elsevier Inc. All rights reserved.
Comment in
- Control of Chromosomal Localization of Xist by hnRNP U Family Molecules.
Sakaguchi T, Hasegawa Y, Brockdorff N, Tsutsui K, Tsutsui KM, Sado T, Nakagawa S. Sakaguchi T, et al. Dev Cell. 2016 Oct 10;39(1):11-12. doi: 10.1016/j.devcel.2016.09.022. Dev Cell. 2016. PMID: 27728779 No abstract available. - SAF-A Requirement in Anchoring XIST RNA to Chromatin Varies in Transformed and Primary Cells.
Kolpa HJ, Fackelmayer FO, Lawrence JB. Kolpa HJ, et al. Dev Cell. 2016 Oct 10;39(1):9-10. doi: 10.1016/j.devcel.2016.09.021. Dev Cell. 2016. PMID: 27728783 Free PMC article. No abstract available.
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