Nitric oxide is associated with long-term zinc tolerance in Solanum nigrum - PubMed (original) (raw)

ROS production in roots of 2-week-old S. nigrum seedlings grown in Hoagland solution exposed to Zn2+ or to Zn2+ plus PTIO or Zn2+ plus

l

-NAME. A, H2O2 production was measured by the DCFH-DA method. Values are normalized against the levels in untreated controls (2 h) that are given a value of 1. B, O2− production was quantified as described in “Materials and Methods.” Symbols are as follows: black diamonds, control; blue crosses, 0.2 m

m

PTIO; purple squares, 0.5 m

m l

-NAME; red triangles, 400 μ

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ZnCl2; green squares, 400 μ

m

ZnCl2 plus 0.2 m

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PTIO; orange diamonds, 400 μ

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ZnCl2 plus 0.5 m

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-NAME. Asterisks indicate values significantly different from those of the roots treated with 400 μ

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ZnCl2 alone: * Student’s t test with P < 0.05. Images of H2O2 and O2− detection are presented in

Supplemental Figures S6

and

S7

, respectively. FW, Fresh weight; Rel. Fluor., relative fluorescence units.