Optogenetic control of heart muscle in vitro and in vivo - PubMed (original) (raw)

. 2010 Nov;7(11):897-900.

doi: 10.1038/nmeth.1512. Epub 2010 Oct 3.

Affiliations

• PMID: 20881965
• DOI: 10.1038/nmeth.1512

Optogenetic control of heart muscle in vitro and in vivo

Tobias Bruegmann et al. Nat Methods. 2010 Nov.

Abstract

Electrical stimulation is the standard technique for exploring electrical behavior of heart muscle, but this approach has considerable technical limitations. Here we report expression of the light-activated cation channel channelrhodopsin-2 for light-induced stimulation of heart muscle in vitro and in mice. This method enabled precise localized stimulation and constant prolonged depolarization of cardiomyocytes and cardiac tissue resulting in alterations of pacemaking, Ca(2+) homeostasis, electrical coupling and arrhythmogenic spontaneous extrabeats.

PubMed Disclaimer

Comment in

• Pacing lightly: optogenetics gets to the heart.
  Knollmann BC. Knollmann BC. Nat Methods. 2010 Nov;7(11):889-91. doi: 10.1038/nmeth1110-889. Nat Methods. 2010. PMID: 21030965 No abstract available.

References

1. 1. Nature. 2007 Dec 6;450(7171):819-24 - PubMed
2. 1. J Gen Physiol. 2007 Aug;130(2):133-44 - PubMed
3. 1. Annu Rev Physiol. 2008;70:23-49 - PubMed
4. 1. Differentiation. 1991 Dec;48(3):173-82 - PubMed
5. 1. Nat Neurosci. 2005 Sep;8(9):1263-8 - PubMed

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Nature Publishing Group

• Other Literature Sources

  • The Lens - Patent Citations

• Molecular Biology Databases

  • Mouse Genome Informatics (MGI)

• Miscellaneous

  • NCI CPTAC Assay Portal