Granada virus: a natural phlebovirus reassortant of the sandfly fever Naples serocomplex with low seroprevalence in humans - PubMed (original) (raw)

Granada virus: a natural phlebovirus reassortant of the sandfly fever Naples serocomplex with low seroprevalence in humans

Ximena Collao et al. Am J Trop Med Hyg. 2010 Oct.

Abstract

A new member of the phlebovirus genus, tentatively named Granada virus, was detected in sandflies collected in Spain. By showing the presence of specific neutralizing antibodies in human serum collected in Granada, we show that Granada virus infects humans. The analysis of the complete genome of Granada virus revealed that this agent is likely to be a natural reassortant of the recently described Massilia virus (donor of the long and short segments) with a yet unidentified phlebovirus (donor of the medium segment).

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Figures

Figure 1.

Figure 1.

Phylogenetic analysis of phlebovirus. Coding regions for nucleoprotein (N) gene, nonstructural (NSs) gene, large (L) segment, and medium (M) segment were studied by using distance MEGA (

www.megasoftware.net

); relationships between different strains are shown. Each sequence used shows the GenBank accession number followed by the name of the virus according to the International Committee on Taxonomy of Viruses and the corresponding strain. RVFV = Rift Valley Fever virus; PTV = Punta Toro virus; SFSV = Sandfly Fever Sicilian virus; UUKV = Uukuniemi virus; SFNV = Sandfly Fever Naples virus; FRIV = Frijoles virus; ICOV = Icoaraci virus; JOAV = Joa virus; BELTV = Belterra virus; SLBOV = Salobo virus; BUEV = Buenaventura virus; TOSV = Toscana virus; GRV = Granada virus; MASV = Massilia virus. Scale bars indicate nucleotide substitutions per site.

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