The impact of individual in vivo repair of DNA double-strand breaks on oral mucositis in adjuvant radiotherapy of head-and-neck cancer - PubMed (original) (raw)
. 2011 Dec 1;81(5):1465-72.
doi: 10.1016/j.ijrobp.2010.08.004. Epub 2010 Oct 13.
Affiliations
- PMID: 20947264
- DOI: 10.1016/j.ijrobp.2010.08.004
The impact of individual in vivo repair of DNA double-strand breaks on oral mucositis in adjuvant radiotherapy of head-and-neck cancer
Jochen Fleckenstein et al. Int J Radiat Oncol Biol Phys. 2011.
Abstract
Purpose: To evaluate the impact of individual in vivo DNA double-strand break (DSB) repair capacity on the incidence of severe oral mucositis in patients with head-and-neck cancer undergoing adjuvant radiotherapy (RT) or radiochemotherapy (RCT).
Patients and methods: Thirty-one patients with resected head-and-neck cancer undergoing adjuvant RT or RCT were examined. Patients underwent RT of the primary tumor site and locoregional lymph nodes with a total dose of 60-66 Gy (single dose 2 Gy, five fractions per week). Chemotherapy consisted of two cycles of cisplatin and 5-fluorouracil. To assess DSB repair, γ-H2AX foci in blood lymphocytes were quantified before and 0.5 h, 2.5 h, 5 h, and 24 h after in vivo radiation exposure (the first fraction of RT). World Health Organization scores for oral mucositis were documented weekly and correlated with DSB repair.
Results: Sixteen patients received RT alone; 15 patients received RCT. In patients who developed Grade≥3 mucositis (n=18) the amount of unrepaired DSBs 24 h after radiation exposure and DSB repair half-times did not differ significantly from patients with Grade≤2 mucositis (n=13). Patients with a proportion of unrepaired DSBs after 24 h higher than the mean value + one standard deviation had an increased incidence of severe oral mucositis.
Conclusions: Evaluation of in vivo DSB repair by determination of γ-H2AX foci loss is feasible in clinical practice and allows identification of patients with impaired DSB repair. The incidence of oral mucositis is not closely correlated with DSB repair under the evaluated conditions.
Copyright © 2011 Elsevier Inc. All rights reserved.
Similar articles
- DNA double-strand break analysis by γ-H2AX foci: a useful method for determining the overreactors to radiation-induced acute reactions among head-and-neck cancer patients.
Goutham HV, Mumbrekar KD, Vadhiraja BM, Fernandes DJ, Sharan K, Kanive Parashiva G, Kapaettu S, Bola Sadashiva SR. Goutham HV, et al. Int J Radiat Oncol Biol Phys. 2012 Dec 1;84(5):e607-12. doi: 10.1016/j.ijrobp.2012.06.041. Epub 2012 Jul 24. Int J Radiat Oncol Biol Phys. 2012. PMID: 22836053 - Dimethyl Sulfoxide Prevents Radiation-Induced Oral Mucositis Through Facilitating DNA Double-Strand Break Repair in Epithelial Stem Cells.
Yang C, Tang H, Wang L, Peng R, Bai F, Shan Y, Yu Z, Zhou P, Cong Y. Yang C, et al. Int J Radiat Oncol Biol Phys. 2018 Dec 1;102(5):1577-1589. doi: 10.1016/j.ijrobp.2018.07.2010. Epub 2018 Aug 6. Int J Radiat Oncol Biol Phys. 2018. PMID: 30092334 - Palifermin decreases severe oral mucositis of patients undergoing postoperative radiochemotherapy for head and neck cancer: a randomized, placebo-controlled trial.
Henke M, Alfonsi M, Foa P, Giralt J, Bardet E, Cerezo L, Salzwimmer M, Lizambri R, Emmerson L, Chen MG, Berger D. Henke M, et al. J Clin Oncol. 2011 Jul 10;29(20):2815-20. doi: 10.1200/JCO.2010.32.4103. Epub 2011 Jun 13. J Clin Oncol. 2011. PMID: 21670447 Clinical Trial. - Mucositis incidence, severity and associated outcomes in patients with head and neck cancer receiving radiotherapy with or without chemotherapy: a systematic literature review.
Trotti A, Bellm LA, Epstein JB, Frame D, Fuchs HJ, Gwede CK, Komaroff E, Nalysnyk L, Zilberberg MD. Trotti A, et al. Radiother Oncol. 2003 Mar;66(3):253-62. doi: 10.1016/s0167-8140(02)00404-8. Radiother Oncol. 2003. PMID: 12742264 Review. - A biological approach to mucositis.
Sonis ST. Sonis ST. J Support Oncol. 2004 Jan-Feb;2(1):21-32; discussion 35-6. J Support Oncol. 2004. PMID: 15330370 Review.
Cited by
- The kinetics of γ-H2AX during radiotherapy of head and neck cancer potentially allow for prediction of severe mucositis.
Kazmierska J, Barczak W, Winiecki T, Łuczewski Ł, Marciniak M, Suchorska W. Kazmierska J, et al. Radiol Oncol. 2020 Feb 14;54(1):96-102. doi: 10.2478/raon-2020-0005. Radiol Oncol. 2020. PMID: 32061167 Free PMC article. - DNA Damage Response Assessments in Human Tumor Samples Provide Functional Biomarkers of Radiosensitivity.
Willers H, Gheorghiu L, Liu Q, Efstathiou JA, Wirth LJ, Krause M, von Neubeck C. Willers H, et al. Semin Radiat Oncol. 2015 Oct;25(4):237-50. doi: 10.1016/j.semradonc.2015.05.007. Epub 2015 May 14. Semin Radiat Oncol. 2015. PMID: 26384272 Free PMC article. Review. - Prediction of the Acute or Late Radiation Toxicity Effects in Radiotherapy Patients Using Ex Vivo Induced Biodosimetric Markers: A Review.
Vinnikov V, Hande MP, Wilkins R, Wojcik A, Zubizarreta E, Belyakov O. Vinnikov V, et al. J Pers Med. 2020 Dec 16;10(4):285. doi: 10.3390/jpm10040285. J Pers Med. 2020. PMID: 33339312 Free PMC article. Review. - Correlation of dynamic changes in γ-H2AX expression in peripheral blood lymphocytes from head and neck cancer patients with radiation-induced oral mucositis.
Li P, Du CR, Xu WC, Shi ZL, Zhang Q, Li ZB, Fu S. Li P, et al. Radiat Oncol. 2013 Jun 26;8:155. doi: 10.1186/1748-717X-8-155. Radiat Oncol. 2013. PMID: 23803252 Free PMC article. - Radiosensitivity in breast cancer assessed by the histone γ-H2AX and 53BP1 foci.
Djuzenova CS, Elsner I, Katzer A, Worschech E, Distel LV, Flentje M, Polat B. Djuzenova CS, et al. Radiat Oncol. 2013 Apr 24;8:98. doi: 10.1186/1748-717X-8-98. Radiat Oncol. 2013. PMID: 23617930 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Medical