Isolation of low-n amyloid β-protein oligomers from cultured cells, CSF, and brain - PubMed (original) (raw)
Isolation of low-n amyloid β-protein oligomers from cultured cells, CSF, and brain
Ganesh M Shankar et al. Methods Mol Biol. 2011.
Abstract
Recent data suggest that soluble, non-fibrillar assemblies of the amyloid β-protein (Aβ) may mediate the synaptic deficits that characterize the early stages of Alzheimer's disease. Consequently, much effort has been expended in isolating and studying a variety of different Aβ assemblies. Here, we describe the use of immunoprecipitation/western blotting and size exclusion chromatography/western blotting to characterize Aβ present in conditioned medium from cultured cells, human cerebrospinal fluid, and human cortex extracted with aqueous buffer, detergent, and formic acid.
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