Stem cell-based biological tooth repair and regeneration - PubMed (original) (raw)

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Stem cell-based biological tooth repair and regeneration

Ana Angelova Volponi et al. Trends Cell Biol. 2010 Dec.

Abstract

Teeth exhibit limited repair in response to damage, and dental pulp stem cells probably provide a source of cells to replace those damaged and to facilitate repair. Stem cells in other parts of the tooth, such as the periodontal ligament and growing roots, play more dynamic roles in tooth function and development. Dental stem cells can be obtained with ease, making them an attractive source of autologous stem cells for use in restoring vital pulp tissue removed because of infection, in regeneration of periodontal ligament lost in periodontal disease, and for generation of complete or partial tooth structures to form biological implants. As dental stem cells share properties with mesenchymal stem cells, there is also considerable interest in their wider potential to treat disorders involving mesenchymal (or indeed non-mesenchymal) cell derivatives, such as in Parkinson's disease.

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Figures

Figure 1

Figure 1

Tooth formation in vitro from combinations of mouse epithelial and mesenchymal cells. The epithelium (red arrow) and mesenchyme (black arrow) are separated from pre-bud stage tooth primordia,and cells dissociated in single cell populations. (a) The cells are recombined (as shown in this figure) and grown in vitro for 6 days. (b) Gross appearance after 9 days in culture with higher magnification of a tooth primordium. (c) Sections of tooth primordia from (a), showing development to the bell stage.

Figure 2

Figure 2

Diagrammatic representation of the generation of biological replacement teeth. Suitable sources of epithelial and mesenchymal cells are expanded in culture to generate sufficient cells. The two cell populations are combined to bring the epithelial and mesenchymal cells into direct contact, mimicking the in vivo arrangement. Interaction between these cell types leads to formation of an early stage tooth primordium, equivalent to a tooth bud or cap, around which the mesenchyme cells condense (dark blue dots) (see also Box 1). The tooth primordium is surgically transplanted into the mouth and left to develop.

Figure I

Figure I

Diagrammatic representation of tooth development.

Figure II

Figure II

Photograph and diagram of a human third molar tooth following extraction. A hemisected tooth showing the internal tissues is shown on the right. Because the tooth was in the process of erupting, root growth is incomplete, and the apical papilla is visible. A diagrammatic representation of this tooth is shown on the left.

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