Effect of Ambrotose AO® on resting and exercise-induced antioxidant capacity and oxidative stress in healthy adults - PubMed (original) (raw)

Randomized Controlled Trial

Effect of Ambrotose AO® on resting and exercise-induced antioxidant capacity and oxidative stress in healthy adults

Richard J Bloomer et al. Nutr J. 2010.

Abstract

Background: The purpose of this investigation was to determine the effects of a dietary supplement (Ambrotose AO®) on resting and exercise-induced blood antioxidant capacity and oxidative stress in exercise-trained and untrained men and women.

Methods: 25 individuals (7 trained and 5 untrained men; 7 trained and 6 untrained women) received Ambrotose AO® (4 capsules per day = 2 grams per day) or a placebo for 3 weeks in a random order, double blind cross-over design (with a 3 week washout period). Blood samples were collected at rest, and at 0 and 30 minutes following a graded exercise treadmill test (GXT) performed to exhaustion, both before and after each 3 week supplementation period. Samples were analyzed for Trolox Equivalent Antioxidant Capacity (TEAC), Oxygen Radical Absorbance Capacity (ORAC), malondialdehyde (MDA), hydrogen peroxide (H2O2), and nitrate/nitrite (NOx). Quality of life was assessed using the SF-12 form and exercise time to exhaustion was recorded. Resting blood samples were analyzed for complete blood count (CBC), metabolic panel, and lipid panel before and after each 3 week supplementation period. Dietary intake during the week before each exercise test was recorded.

Results: No condition effects were noted for SF-12 data, for GXT time to exhaustion, or for any variable within the CBC, metabolic panel, or lipid panel (p > 0.05). Treatment with Ambrotose AO® resulted in an increase in resting levels of TEAC (p = 0.02) and ORAC (p < 0.0001). No significant change was noted in resting levels of MDA, H2O2, or NOx (p > 0.05). Exercise resulted in an acute increase in TEAC, MDA, and H2O2 (p < 0.05), all which were higher at 0 minutes post exercise compared to pre exercise (p < 0.05). No condition effects were noted for exercise related data (p > 0.05), with the exception of ORAC (p = 0.0005) which was greater at 30 minutes post exercise for Ambrotose AO® compared to placebo.

Conclusion: Ambrotose AO® at a daily dosage of 4 capsules per day increases resting blood antioxidant capacity and may enhance post exercise antioxidant capacity. However, no statistically detected difference is observed in resting or exercise-induced oxidative stress biomarkers, in quality of life, or in GXT time to exhaustion.

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Figures

Figure 1

Figure 1

Timeline of study to investigate the effect of Ambrotose AO® on resting and exercise-induced antioxidant capacity and oxidative stress in healthy adults.

Figure 2

Figure 2

Serum Trolox Equivalent Antioxidant Capacity (TEAC) and Oxygen Radical Absorbance Capacity (ORAC) of 25 subjects (12 men and 13 women) before and following three weeks of supplementation with Ambrotose AO® at a dosage of 4 capsules per day and placebo (cross-over design with a three week washout between conditions). Values are mean ± SEM. For TEAC: Condition × time interaction (p = 0.17) * Paired contrast between pre and post intervention for Ambrotose AO® (p = 0.02) For ORAC: Condition × time interaction (p = 0.01) * Paired contrast between pre and post intervention for Ambrotose AO® (p < 0.0001)

Figure 3

Figure 3

Plasma Malondialdehyde (MDA), Hydrogen Peroxide (H2O2), and Nitrate/Nitrite (NOx) of 25 subjects (12 men and 13 women) before and following three weeks of supplementation with Ambrotose AO® at a dosage of 4 capsules per day and placebo (cross-over design with a three week washout between conditions). Values are mean ± SEM. For MDA: Condition × time interaction (p = 0.77) Paired contrast between pre and post intervention for Ambrotose AO® (p = 0.61) For H2O2: Condition × time interaction (p = 0.53) Paired contrast between pre and post intervention for Ambrotose AO® (p = 0.41) For NOx: Condition × time interaction (p = 0.11) Paired contrast between pre and post intervention for Ambrotose AO® (p = 0.12)

Figure 4

Figure 4

Serum Trolox Equivalent Antioxidant Capacity (TEAC) and Oxygen Radical Absorbance Capacity (ORAC) of 25 subjects (12 men and 13 women) before and at 0 and 30 minutes after a graded exercise treadmill test to exhaustion, before and following three weeks of supplementation with Ambrotose AO® at a dosage of 4 capsules per day and placebo (cross-over design with a three week washout between conditions). Values are mean ± SEM. For TEAC: *Time effect (p = 0.02) For ORAC: Condition × time interaction (p < 0.0001) * Paired contrast between Ambrotose AO® and placebo (p < 0.0001)

Figure 5

Figure 5

Plasma Malondialdehyde (MDA), Hydrogen Peroxide (H 2 O 2 ), and Nitrate/Nitrite (NOx) of 25 subjects (12 men and 13 women) before and at 0 and 30 minutes after a graded exercise treadmill test to exhaustion, before and following three weeks of supplementation with Ambrotose AO® at a dosage of 4 capsules per day and placebo (cross-over design with a three week washout between conditions). Values are mean ± SEM. For MDA: *Time effect (p = 0.05) For H2O2: *Time effect (p < 0.0001)

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