TNF-alpha antagonism with etanercept decreases glucose and increases the proportion of high molecular weight adiponectin in obese subjects with features of the metabolic syndrome - PubMed (original) (raw)
Randomized Controlled Trial
. 2011 Jan;96(1):E146-50.
doi: 10.1210/jc.2010-1170. Epub 2010 Nov 3.
Affiliations
- PMID: 21047923
- PMCID: PMC3038481
- DOI: 10.1210/jc.2010-1170
Randomized Controlled Trial
TNF-alpha antagonism with etanercept decreases glucose and increases the proportion of high molecular weight adiponectin in obese subjects with features of the metabolic syndrome
Takara L Stanley et al. J Clin Endocrinol Metab. 2011 Jan.
Abstract
Context and objective: Obesity is associated with activation of the TNF-α system, increased inflammatory markers, and insulin resistance. Although studies in rodents suggest that attenuation of TNF activity improves glucose homeostasis, the effect of prolonged inhibition of TNF-α with etanercept on inflammation and glucose homeostasis in a human model of obesity is not known.
Design and participants: Forty obese subjects with features of metabolic syndrome were randomized to etanercept or placebo, 50 mg twice weekly for 3 months, followed by 50 mg once weekly for 3 months.
Outcome measures: Subjects underwent oral glucose tolerance testing and measurement of serum inflammatory biomarkers and adipokines. Subcutaneous fat biopsy was performed in a subset for measurement of adipokine and TNF-α mRNA expression.
Results: Visceral adiposity was significantly associated with serum concentrations of TNF receptor 1 (TNFR1), TNFR2, and vascular cell adhesion molecule-1 and adipose tissue expression of TNF-α and SOCS-3 (all P < 0.05). Insulin resistance as assessed by homeostasis model assessment was significantly associated with TNFR1, C-reactive protein, IL-6, and soluble intracellular adhesion molecule-1 (sICAM-1) (all P < 0.05). Etanercept significantly improved fasting glucose (treatment effect vs. placebo over 6 months, -10.8 ± 4.4%, P = 0.02). Etanercept also increased the ratio of high molecular weight adiponectin to total adiponectin (+22.1 ± 9.2% vs. placebo, P = 0.02), and decreased levels of sICAM-1 (-11 ± 2% vs. placebo, P < 0.0001). In contrast, body composition, lipids, C-reactive protein, and IL-6 were unchanged after 6 months.
Conclusions: Prolonged therapy with etanercept improved fasting glucose, increased the ratio of high molecular weight to total adiponectin, and decreased sICAM-1 in obese subjects with abnormal glucose homeostasis and significant subclinical inflammation.
Figures
Figure 1
Effect of etanercept on glucose, hsCRP, and adiponectin: changes in etanercept (black bars) and placebo (white bars) groups. Error bars are
se
. A, Percent change in fasting glucose at 3 and 6 months; P = 0.02 for etanercept vs. placebo by repeated-measures ANCOVA. B, Change in log10 hsCRP at 3 and 6 months. *, P = 0.03 for change in etanercept vs. placebo at 3 months controlling for age and race. Change between groups is not significantly different at 6 months. C, Aggregate percent change in total adiponectin, HMW adiponectin, and ratio of HMW to total adiponectin over 6 months. *, P < 0.05 for etanercept vs. placebo by repeated-measures ANCOVA.
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