Higher levels of cervicovaginal inflammatory and regulatory cytokines and chemokines in healthy young women with immature cervical epithelium - PubMed (original) (raw)

Higher levels of cervicovaginal inflammatory and regulatory cytokines and chemokines in healthy young women with immature cervical epithelium

Loris Y Hwang et al. J Reprod Immunol. 2011 Jan.

Abstract

Young women aged 15-24 years have the highest rates of sexually transmitted infections (STIs). The vulnerability of adolescents is often attributed to risky sexual behaviors, whereas biological factors affecting mucosal immunity are poorly understood. The objective of this cross-sectional study was to examine associations between the type of cervical epithelium and protein levels of 11 cervicovaginal cytokines and chemokines in non-pregnant healthy young women. Cervical epithelial types were viewed on colpophotography and measured quantitatively using computerized planimetry. We selected 16 women with immature epithelium (predominantly columnar and early/mid squamous metaplasia), and 16 women with mature epithelium (predominantly squamous epithelium). Cytokine levels were measured in cervicovaginal lavage samples by MILLIPLEX™ MAP Human Cytokine/Chemokine multiplex immunoassay. Bivariate Box-Cox regression models compared cytokine levels between immature and mature groups. Multivariate Box-Cox models adjusted separately for age, years since menarche, days since last menses, years of sexual activity, number of lifetime sexual partners, HPV infection, hormonal contraceptive use, smoking, bacterial vaginosis by Nugent's criteria, and polymorphonuclear cells on wet prep. The mean age was 19.2 years. Women with immature epithelium demonstrated significantly higher levels of IL-1α, IL-1β, IL-6, IL-8, MIP-1α, RANTES, TNFα, IL-10, IL-12 and IFNγ (each p<0.01), compared to women with mature epithelium. Results remained highly significant in the multivariate models. Cytokine profiles in the healthy state may foreshadow differential responses to pathogens. Cervical epithelial type should be measured in clinical studies involving cervicovaginal immune markers.

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Figures

Figure 1

Quantitative measurements of the cervix performed by colpophotography and computerized planimetry. Cervical immaturity was defined as areas of columnar and early-mid squamous metaplasia and calculated as a percentage of the total cervical face. (A) Example of cervical immaturity measured as 67% of the total cervical face. (B) Example of cervical immaturity measured as <5% of the total cervical face.

Figure 1

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References

1. Amsel R, Totten PA, Spiegel CA, Chen KC, Eschenbach D, Homes KK. Nonspecific vaginitis. Diagnostic criteria and microbial and epidemiologic associations. Am J Med. 1983;74:14–22. - PubMed
1. Box GEP, Cox DR. An analysis of transformations. J R Stat Soc Ser B (Methodological) 1964;26:211–46.
1. Brunham RC, Peeling RW. Chlamydia trachomatis antigens: Role in immunity and pathogenesis. Infect Agents Dis. 1994;3:218–33. - PubMed
1. Centers for Disease Control and Prevention. Sexually transmitted disease surveillance, 2008. U.S. Department of Health and Human Services; Atlanta, Georgia: 2009.
1. Fahey JV, Schaefer TM, Channon JY, Wira CR. Secretion of cytokines and chemokines by polarized human epithelial cells from the female reproductive tract. Hum Reprod. 2005;20:1439–46. - PubMed

Higher levels of cervicovaginal inflammatory and regulatory cytokines and chemokines in healthy young women with immature cervical epithelium - PubMed (original) (raw)