Epiblast stem cell subpopulations represent mouse embryos of distinct pregastrulation stages - PubMed (original) (raw)

. 2010 Nov 12;143(4):617-27.

doi: 10.1016/j.cell.2010.10.015.

Affiliations

• PMID: 21056461
• DOI: 10.1016/j.cell.2010.10.015

Free article

Epiblast stem cell subpopulations represent mouse embryos of distinct pregastrulation stages

Dong Wook Han et al. Cell. 2010.

Free article

Abstract

Embryonic stem cells (ESCs) comprise at least two populations of cells with divergent states of pluripotency. Here, we show that epiblast stem cells (EpiSCs) also comprise two distinct cell populations that can be distinguished by the expression of a specific Oct4-GFP marker. These two subpopulations, Oct4-GFP positive and negative EpiSCs, are capable of converting into each other in vitro. Oct4-GFP positive and negative EpiSCs are distinct from ESCs with respect to global gene expression pattern, epigenetic profile, and Oct4 enhancer utilization. Oct4-GFP negative cells share features with cells of the late mouse epiblast and cannot form chimeras. However, Oct4-GFP positive EpiSCs, which only represent a minor EpiSC fraction, resemble cells of the early epiblast and can readily contribute to chimeras. Our findings suggest that the rare ability of EpiSCs to contribute to chimeras is due to the presence of the minor EpiSC fraction representing the early epiblast.

Copyright © 2010 Elsevier Inc. All rights reserved.

PubMed Disclaimer

Comment in

• Another horse in the meta-stable state of pluripotency.
  Cherry A, Daley GQ. Cherry A, et al. Cell Stem Cell. 2010 Dec 3;7(6):641-2. doi: 10.1016/j.stem.2010.11.020. Cell Stem Cell. 2010. PMID: 21112555 No abstract available.

Similar articles

• Distinct developmental ground states of epiblast stem cell lines determine different pluripotency features.
  Bernemann C, Greber B, Ko K, Sterneckert J, Han DW, Araúzo-Bravo MJ, Schöler HR. Bernemann C, et al. Stem Cells. 2011 Oct;29(10):1496-503. doi: 10.1002/stem.709. Stem Cells. 2011. PMID: 21898681
• Efficient derivation of bovine embryonic stem cells needs more than active core pluripotency factors.
  Maruotti J, Muñoz M, Degrelle SA, Gómez E, Louet C, Díez C, de Longchamp PH, Brochard V, Hue I, Caamaño JN, Jouneau A. Maruotti J, et al. Mol Reprod Dev. 2012 Jul;79(7):461-77. doi: 10.1002/mrd.22051. Epub 2012 May 31. Mol Reprod Dev. 2012. PMID: 22573702
• Neural stem cells derived from epiblast stem cells display distinctive properties.
  Jang HJ, Kim JS, Choi HW, Jeon I, Choi S, Kim MJ, Song J, Do JT. Jang HJ, et al. Stem Cell Res. 2014 Mar;12(2):506-16. doi: 10.1016/j.scr.2013.12.012. Epub 2014 Jan 4. Stem Cell Res. 2014. PMID: 24463498
• Epiblast stem cells contribute new insight into pluripotency and gastrulation.
  Chenoweth JG, McKay RD, Tesar PJ. Chenoweth JG, et al. Dev Growth Differ. 2010 Apr;52(3):293-301. doi: 10.1111/j.1440-169X.2010.01171.x. Epub 2010 Mar 7. Dev Growth Differ. 2010. PMID: 20298258 Review.
• Chicken embryonic stem cells as a non-mammalian embryonic stem cell model.
  Lavial F, Pain B. Lavial F, et al. Dev Growth Differ. 2010 Jan;52(1):101-14. doi: 10.1111/j.1440-169X.2009.01152.x. Epub 2009 Dec 20. Dev Growth Differ. 2010. PMID: 20039925 Review.

Cited by

• Sirtuin 1 Promotes Deacetylation of Oct4 and Maintenance of Naive Pluripotency.
  Williams EO, Taylor AK, Bell EL, Lim R, Kim DM, Guarente L. Williams EO, et al. Cell Rep. 2016 Oct 11;17(3):809-820. doi: 10.1016/j.celrep.2016.09.046. Cell Rep. 2016. PMID: 27732856 Free PMC article.
• Pluripotency in the embryo and in culture.
  Nichols J, Smith A. Nichols J, et al. Cold Spring Harb Perspect Biol. 2012 Aug 1;4(8):a008128. doi: 10.1101/cshperspect.a008128. Cold Spring Harb Perspect Biol. 2012. PMID: 22855723 Free PMC article. Review.
• Capturing Transitional Pluripotency through Proline Metabolism.
  Minchiotti G, D'Aniello C, Fico A, De Cesare D, Patriarca EJ. Minchiotti G, et al. Cells. 2022 Jul 6;11(14):2125. doi: 10.3390/cells11142125. Cells. 2022. PMID: 35883568 Free PMC article. Review.
• A modified EpiSC culture condition containing a GSK3 inhibitor can support germline-competent pluripotency in mice.
  Tsukiyama T, Ohinata Y. Tsukiyama T, et al. PLoS One. 2014 Apr 15;9(4):e95329. doi: 10.1371/journal.pone.0095329. eCollection 2014. PLoS One. 2014. PMID: 24736627 Free PMC article.
• Transcription factor heterogeneity and epiblast pluripotency.
  Osorno R, Chambers I. Osorno R, et al. Philos Trans R Soc Lond B Biol Sci. 2011 Aug 12;366(1575):2230-7. doi: 10.1098/rstb.2011.0043. Philos Trans R Soc Lond B Biol Sci. 2011. PMID: 21727128 Free PMC article.

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Elsevier Science

• Other Literature Sources

  • The Lens - Patent Citations Database

• Medical

  • MedlinePlus Health Information

• Molecular Biology Databases

  • Mouse Genome Informatics (MGI)
  • NIAID Data Ecosystem - Find datasets on Infectious and Immune-mediated Diseases