Toward global RNA structure analysis - PubMed (original) (raw)

Comment

Toward global RNA structure analysis

David M Mauger et al. Nat Biotechnol. 2010 Nov.

No abstract available

Figures

Figure 1

Overview of the PARS approach for measuring RNA structure on a transcriptome scale. RNAs are refolded in vitro, cleaved with structure-selective ribonucleases, fragmented and subjected to highly parallel sequencing. The PARS score gives a measure of double versus single strandedness. One use of the PARS scores is to average these values over many RNAs to yield a global view of RNA structure. PARS suggests that most S. cerevisiae transcripts have less secondary structure in untranslated regions (UTRs) than in coding regions, and that mRNAs with structured translation start sites are translated with lower efficiency than those with unstructured translation start sites.

Comment on

Genome-wide measurement of RNA secondary structure in yeast.
Kertesz M, Wan Y, Mazor E, Rinn JL, Nutter RC, Chang HY, Segal E. Kertesz M, et al. Nature. 2010 Sep 2;467(7311):103-7. doi: 10.1038/nature09322. Nature. 2010. PMID: 20811459 Free PMC article.

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Toward global RNA structure analysis - PubMed (original) (raw)