Physical aspects of COPI vesicle formation - PubMed (original) (raw)

Review

Physical aspects of COPI vesicle formation

Mathieu Pinot et al. Mol Membr Biol. 2010 Nov.

Free article

Abstract

Coat proteins orchestrate membrane budding and molecular sorting during the formation of transport intermediates. Coat protein complex I (COPI) vesicles shuttle between the Golgi apparatus and the endoplasmic reticulum and between Golgi stacks. The formation of a COPI vesicle proceeds in four steps: coat self-assembly, membrane deformation into a bud, fission of the coated vesicle and final disassembly of the coat to ensure recycling of coat components. Although some issues are still actively debated, the molecular mechanisms of COPI vesicle formation are now fairly well understood. In this review, we argue that physical parameters are critical regulators of COPI vesicle formation. We focus on recent real-time in vitro assays highlighting the role of membrane tension, membrane composition, membrane curvature and lipid packing in membrane remodelling and fission by the COPI coat.

PubMed Disclaimer

Similar articles

• Functional reconstitution of COPI coat assembly and disassembly using chemically defined components.
  Reinhard C, Schweikert M, Wieland FT, Nickel W. Reinhard C, et al. Proc Natl Acad Sci U S A. 2003 Jul 8;100(14):8253-7. doi: 10.1073/pnas.1432391100. Epub 2003 Jun 27. Proc Natl Acad Sci U S A. 2003. PMID: 12832619 Free PMC article.
• Lipid packing sensed by ArfGAP1 couples COPI coat disassembly to membrane bilayer curvature.
  Bigay J, Gounon P, Robineau S, Antonny B. Bigay J, et al. Nature. 2003 Dec 4;426(6966):563-6. doi: 10.1038/nature02108. Nature. 2003. PMID: 14654841
• COPI coat assembly occurs on liquid-disordered domains and the associated membrane deformations are limited by membrane tension.
  Manneville JB, Casella JF, Ambroggio E, Gounon P, Bertherat J, Bassereau P, Cartaud J, Antonny B, Goud B. Manneville JB, et al. Proc Natl Acad Sci U S A. 2008 Nov 4;105(44):16946-51. doi: 10.1073/pnas.0807102105. Epub 2008 Oct 30. Proc Natl Acad Sci U S A. 2008. PMID: 18974217 Free PMC article.
• The COPI system: molecular mechanisms and function.
  Beck R, Rawet M, Wieland FT, Cassel D. Beck R, et al. FEBS Lett. 2009 Sep 3;583(17):2701-9. doi: 10.1016/j.febslet.2009.07.032. Epub 2009 Jul 22. FEBS Lett. 2009. PMID: 19631211 Review.
• COPI budding within the Golgi stack.
  Popoff V, Adolf F, Brügger B, Wieland F. Popoff V, et al. Cold Spring Harb Perspect Biol. 2011 Nov 1;3(11):a005231. doi: 10.1101/cshperspect.a005231. Cold Spring Harb Perspect Biol. 2011. PMID: 21844168 Free PMC article. Review.

Cited by

• The Energy of COPI for Budding Membranes.
  Thiam AR, Pincet F. Thiam AR, et al. PLoS One. 2015 Jul 28;10(7):e0133757. doi: 10.1371/journal.pone.0133757. eCollection 2015. PLoS One. 2015. PMID: 26218078 Free PMC article.
• P4 ATPases: flippases in health and disease.
  van der Mark VA, Elferink RP, Paulusma CC. van der Mark VA, et al. Int J Mol Sci. 2013 Apr 11;14(4):7897-922. doi: 10.3390/ijms14047897. Int J Mol Sci. 2013. PMID: 23579954 Free PMC article. Review.
• Dopamine reduces cell surface Na+/H+ exchanger-3 protein by decreasing NHE3 exocytosis and cell membrane recycling.
  Hu MC, Bobulescu IA, Quiñones H, Gisler SM, Moe OW. Hu MC, et al. Am J Physiol Renal Physiol. 2017 Oct 1;313(4):F1018-F1025. doi: 10.1152/ajprenal.00251.2017. Epub 2017 Aug 2. Am J Physiol Renal Physiol. 2017. PMID: 28768665 Free PMC article.
• Reconstitution and real-time quantification of membrane remodeling by single proteins and protein complexes.
  Bashkirov PV, Kuzmin PI, Chekashkina K, Arrasate P, Vera Lillo J, Shnyrova AV, Frolov VA. Bashkirov PV, et al. Nat Protoc. 2020 Aug;15(8):2443-2469. doi: 10.1038/s41596-020-0337-1. Epub 2020 Jun 26. Nat Protoc. 2020. PMID: 32591769 Free PMC article.
• Expression of Rab25 correlates with the invasion and metastasis of gastric cancer.
  Cao C, Lu C, Xu J, Zhang J, Zhang J, Li M. Cao C, et al. Chin J Cancer Res. 2013 Apr;25(2):192-9. doi: 10.3978/j.issn.1000-9604.2013.03.01. Chin J Cancer Res. 2013. PMID: 23592900 Free PMC article.

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Atypon