Dominant and recessive alleles of the Drosophila easter gene are point mutations at conserved sites in the serine protease catalytic domain - PubMed (original) (raw)
Dominant and recessive alleles of the Drosophila easter gene are point mutations at conserved sites in the serine protease catalytic domain
Y S Jin et al. Cell. 1990.
Abstract
The easter gene, required for the development of all lateral and ventral pattern elements in the Drosophila embryo, appears to encode an extracellular serine protease. Dominant easter alleles increase the number of cells that give rise to lateral and ventral structures. We have found that nine dominant and four recessive mutations are caused by single amino acid substitutions at conserved sites in the putative serine protease catalytic domain. The activity of dominant products was assayed by injecting in vitro synthesized transcripts from the dominant alleles into young embryos. The results suggest that the dominant easter products cleave the normal substrate, but fail to respond to a spatially asymmetric regulator.
Similar articles
- The role of easter, an apparent serine protease, in organizing the dorsal-ventral pattern of the Drosophila embryo.
Chasan R, Anderson KV. Chasan R, et al. Cell. 1989 Feb 10;56(3):391-400. doi: 10.1016/0092-8674(89)90242-0. Cell. 1989. PMID: 2492450 - The Drosophila Stubble-stubbloid gene encodes an apparent transmembrane serine protease required for epithelial morphogenesis.
Appel LF, Prout M, Abu-Shumays R, Hammonds A, Garbe JC, Fristrom D, Fristrom J. Appel LF, et al. Proc Natl Acad Sci U S A. 1993 Jun 1;90(11):4937-41. doi: 10.1073/pnas.90.11.4937. Proc Natl Acad Sci U S A. 1993. PMID: 7685111 Free PMC article. - Regulation of Easter activity is required for shaping the Dorsal gradient in the Drosophila embryo.
Chang AJ, Morisato D. Chang AJ, et al. Development. 2002 Dec;129(24):5635-45. doi: 10.1242/dev.00161. Development. 2002. PMID: 12421704 - Structure and function of eukaryotic proprotein processing enzymes of the subtilisin family of serine proteases.
Van de Ven WJ, Roebroek AJ, Van Duijnhoven HL. Van de Ven WJ, et al. Crit Rev Oncog. 1993;4(2):115-36. Crit Rev Oncog. 1993. PMID: 8420571 Review. - Adaptive evolution: the struggle for dominance.
Charlesworth B. Charlesworth B. Curr Biol. 1998 Jul 2;8(14):R502-4. doi: 10.1016/s0960-9822(98)70318-5. Curr Biol. 1998. PMID: 9663386 Review.
Cited by
- Cell-specific accumulation of Drosophila proteasomes (MCP) during early development.
Klein U, Gernold M, Kloetzel PM. Klein U, et al. J Cell Biol. 1990 Dec;111(6 Pt 1):2275-82. doi: 10.1083/jcb.111.6.2275. J Cell Biol. 1990. PMID: 2126012 Free PMC article. - Isolation of secreted proteins from Drosophila ovaries and embryos through in vivo BirA-mediated biotinylation.
Stevens LM, Zhang Y, Volnov Y, Chen G, Stein DS. Stevens LM, et al. PLoS One. 2019 Oct 28;14(10):e0219878. doi: 10.1371/journal.pone.0219878. eCollection 2019. PLoS One. 2019. PMID: 31658274 Free PMC article. - A family of serine protease genes expressed in adult buffalo fly (Haematobia irritans exigua).
Elvin CM, Whan V, Riddles PW. Elvin CM, et al. Mol Gen Genet. 1993 Jul;240(1):132-9. doi: 10.1007/BF00276892. Mol Gen Genet. 1993. PMID: 8341258 - Maternal control of the Drosophila dorsal-ventral body axis.
Stein DS, Stevens LM. Stein DS, et al. Wiley Interdiscip Rev Dev Biol. 2014 Sep-Oct;3(5):301-30. doi: 10.1002/wdev.138. Epub 2014 May 29. Wiley Interdiscip Rev Dev Biol. 2014. PMID: 25124754 Free PMC article. Review. - The thick veins gene of Drosophila is required for dorsoventral polarity of the embryo.
Terracol R, Lengyel JA. Terracol R, et al. Genetics. 1994 Sep;138(1):165-78. doi: 10.1093/genetics/138.1.165. Genetics. 1994. PMID: 8001784 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Medical
Molecular Biology Databases