tRNADB-CE 2011: tRNA gene database curated manually by experts - PubMed (original) (raw)

. 2011 Jan;39(Database issue):D210-3.

doi: 10.1093/nar/gkq1007. Epub 2010 Nov 11.

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tRNADB-CE 2011: tRNA gene database curated manually by experts

Takashi Abe et al. Nucleic Acids Res. 2011 Jan.

Abstract

We updated the tRNADB-CE by analyzing 939 complete and 1301 draft genomes of prokaryotes and eukaryotes, 171 complete virus genomes, 121 complete chloroplast genomes and approximately 230 million sequences obtained by metagenome analyses of 210 environmental samples. The 287 102 tRNA genes in total, and thus two times of the tRNA genes compiled previously, are compiled, in which sequence information, clover-leaf structure and results of sequence similarity and oligonucleotide-pattern search can be browsed. In order to pool collective knowledge with help from any experts in the tRNA research field, we included a column to which comments can be added on each tRNA gene. By compiling tRNAs of known prokaryotes with identical sequences, we found high phylogenetic preservation of tRNA sequences, especially at a phylum level. Furthermore, a large number of tRNAs obtained by metagenome analyses of environmental samples had sequences identical to those found in known prokaryotes. The identical sequence group, therefore, can be used as phylogenetic markers to clarify the microbial community structure of an ecosystem. The updated tRNADB-CE provided functions, with which users can obtain the phylotype-specific markers (e.g. genus-specific markers) by themselves and clarify microbial community structures of ecosystems in detail. tRNADB-CE can be accessed freely at http://trna.nagahama-i-bio.ac.jp.

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Figures

Figure 1.

Figure 1.

Basic functions of tRNADB-CE. (a) Phylum/class/species list and anticodon table, (b) tRNA gene list, (c) Advanced pattern search against each stem/loop and (d) Detail information.

Figure 2.

Figure 2.

List and search for (a) ISG, (b) ISGs for each phylotype, (c) ISGs for each anticodon, (d) ISG tRNAs for one anticodon and (e) ISGs found in an environmental sample.

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