Atlas of gene expression in the mouse kidney: new features of glomerular parietal cells - PubMed (original) (raw)
Atlas of gene expression in the mouse kidney: new features of glomerular parietal cells
Lydie Cheval et al. Physiol Genomics. 2011.
Abstract
To gain molecular insight into kidney function, we performed a high-resolution quantitative analysis of gene expression in glomeruli and nine different nephron segments dissected from mouse kidney using Serial Analysis of Gene Expression (SAGE). We also developed dedicated bioinformatics tools and databases to annotate mRNA tags as transcripts. Over 800,000 mRNA SAGE tags were sequenced corresponding to >20,000 different mRNA tags present at least twice in at least one library. Hierarchical clustering analysis of tags demonstrated similarities between the three anatomical subsegments of the proximal tubule, between the cortical and medullary segments of the thick ascending limb of Henle's loop, and between the three segments constituting the aldosterone-sensitive distal nephron segments, whereas the glomerulus and distal convoluted tubule clusterized independently. We also identified highly specific mRNA markers of each subgroup of nephron segments and of most nephron segments. Tag annotation also identified numbers of putative antisense mRNAs. This database constitutes a reference resource in which the quantitative expression of a given gene can be compared with that of other genes in the same nephron segment, or between different segments of the nephron. To illustrate possible applications of this database, we performed a deeper analysis of the glomerulus transcriptome that unexpectedly revealed expression of several ion and water carriers; within the glomerulus, they were found to be preferentially expressed in the parietal sheet. It also revealed the major role of the zinc finger transcription factor Wt1 in the specificity of gene expression in the glomerulus. Finally, functional annotation of glomerulus-specific transcripts suggested a high proliferation activity of glomerular cells. Immunolabeling for PCNA confirmed a high percentage of proliferating cells in the glomerulus parietal sheet.
Similar articles
- [Transcriptomes for serial analysis of gene expression].
Marti J, Piquemal D, Manchon L, Commes T. Marti J, et al. J Soc Biol. 2002;196(4):303-7. J Soc Biol. 2002. PMID: 12645300 Review. French. - Distribution of thromboxane A2 receptor mRNA along the mouse nephron segments.
Asano K, Taniguchi S, Nakao A, Maruyama T, Watanabe T, Kurokawa K. Asano K, et al. Biochem Biophys Res Commun. 1996 Sep 24;226(3):613-7. doi: 10.1006/bbrc.1996.1404. Biochem Biophys Res Commun. 1996. PMID: 8831666 - A panoramic view of gene expression in the human kidney.
Chabardès-Garonne D, Mejéan A, Aude JC, Cheval L, Di Stefano A, Gaillard MC, Imbert-Teboul M, Wittner M, Balian C, Anthouard V, Robert C, Ségurens B, Wincker P, Weissenbach J, Doucet A, Elalouf JM. Chabardès-Garonne D, et al. Proc Natl Acad Sci U S A. 2003 Nov 11;100(23):13710-5. doi: 10.1073/pnas.2234604100. Epub 2003 Oct 31. Proc Natl Acad Sci U S A. 2003. PMID: 14595018 Free PMC article. - Plasticity of mouse renal collecting duct in response to potassium depletion.
Cheval L, Duong Van Huyen JP, Bruneval P, Verbavatz JM, Elalouf JM, Doucet A. Cheval L, et al. Physiol Genomics. 2004 Sep 16;19(1):61-73. doi: 10.1152/physiolgenomics.00055.2004. Epub 2004 Jul 6. Physiol Genomics. 2004. PMID: 15238618 - Renal transcriptomes: segmental analysis of differential expression.
Elalouf JM, Aude JC, Billon E, Cheval L, Doucet A, Virlon B. Elalouf JM, et al. Exp Nephrol. 2002;10(2):75-81. doi: 10.1159/000049902. Exp Nephrol. 2002. PMID: 11937754 Review.
Cited by
- Lipidomic Profiling of Kidney Cortical Tubule Segments Identifies Lipotypes with Physiological Implications.
Cheval L, Poindessous V, Sampaio JL, Crambert G, Pallet N. Cheval L, et al. Function (Oxf). 2024 Jul 11;5(4):zqae016. doi: 10.1093/function/zqae016. Function (Oxf). 2024. PMID: 38985001 Free PMC article. - Gene expression databases for kidney epithelial cells.
Huling JC, Pisitkun T, Song JH, Yu MJ, Hoffert JD, Knepper MA. Huling JC, et al. Am J Physiol Renal Physiol. 2012 Feb 15;302(4):F401-7. doi: 10.1152/ajprenal.00457.2011. Epub 2011 Nov 23. Am J Physiol Renal Physiol. 2012. PMID: 22114206 Free PMC article. Review. - NDFIP allows NEDD4/NEDD4L-induced AQP2 ubiquitination and degradation.
Trimpert C, Wesche D, de Groot T, Pimentel Rodriguez MM, Wong V, van den Berg DTM, Cheval L, Ariza CA, Doucet A, Stagljar I, Deen PMT. Trimpert C, et al. PLoS One. 2017 Sep 20;12(9):e0183774. doi: 10.1371/journal.pone.0183774. eCollection 2017. PLoS One. 2017. PMID: 28931009 Free PMC article. - Methodology optimizing SAGE library tag-to-gene mapping: application to Leishmania.
Smandi S, Guerfali FZ, Farhat M, Ben-Aissa K, Laouini D, Guizani-Tabbane L, Dellagi K, Benkahla A. Smandi S, et al. BMC Res Notes. 2012 Jan 27;5:74. doi: 10.1186/1756-0500-5-74. BMC Res Notes. 2012. PMID: 22283878 Free PMC article. - Uromodulin: from physiology to rare and complex kidney disorders.
Devuyst O, Olinger E, Rampoldi L. Devuyst O, et al. Nat Rev Nephrol. 2017 Sep;13(9):525-544. doi: 10.1038/nrneph.2017.101. Epub 2017 Aug 7. Nat Rev Nephrol. 2017. PMID: 28781372 Review.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Molecular Biology Databases
Miscellaneous