How to detect NDM-1 producers - PubMed (original) (raw)
Review
How to detect NDM-1 producers
Patrice Nordmann et al. J Clin Microbiol. 2011 Feb.
Abstract
Enterobacterial isolates expressing the carbapenemase NDM-1 are emerging worldwide. Twenty-seven NDM-1-positive isolates of worldwide origin were included in this study to identify these strains as not only pathogens but also colonizers of normal flora for infection control screening. Although susceptibility to carbapenems varied, a combined test (IMP/IMP + EDTA), the Etest MBL, and automated susceptibility testing by Vitek2 (bioMérieux) identified those NDM-1 producers as verified by PCR using specific primers. Screening for carriers of NDM-1 producers may be based on media such as the ChromID ESBL culture medium routinely used to screen for extended-spectrum β-lactamase producers, which gives excellent detection levels with low limits of detection ranging from 8 × 10(0) to 5 × 10(2) CFU/ml. The CHROMagar KPC culture medium had higher limits of detection (1 × 10(1) to 5 × 10(5) CFU/ml) and may be proposed for the follow-up of outbreaks of infections with NDM-1 producers. Colonies growing on these screening media can be verified as NDM-1 producers with molecular methods as described herein.
Figures
Fig. 1.
Strategy for identification of NDM-1 producers as a source of clinical infections and for detecting carriers of NDM-1 producers. *, this culture medium can be used for surveillance of outbreaks of infections with NDM-1 producers after validation of its detection sensitivity for the specific strain responsible for an outbreak. **, Etest MBL is reliable when the MIC of imipenem is not too low.
Similar articles
- A simple phenotypic method for the differentiation of metallo-beta-lactamases and class A KPC carbapenemases in Enterobacteriaceae clinical isolates.
Tsakris A, Poulou A, Pournaras S, Voulgari E, Vrioni G, Themeli-Digalaki K, Petropoulou D, Sofianou D. Tsakris A, et al. J Antimicrob Chemother. 2010 Aug;65(8):1664-71. doi: 10.1093/jac/dkq210. Epub 2010 Jun 11. J Antimicrob Chemother. 2010. PMID: 20542902 - Identification and screening of carbapenemase-producing Enterobacteriaceae.
Nordmann P, Gniadkowski M, Giske CG, Poirel L, Woodford N, Miriagou V; European Network on Carbapenemases. Nordmann P, et al. Clin Microbiol Infect. 2012 May;18(5):432-8. doi: 10.1111/j.1469-0691.2012.03815.x. Clin Microbiol Infect. 2012. PMID: 22507110 Review. - Emergence of NDM-1- and IMP-14a-producing Enterobacteriaceae in Thailand.
Rimrang B, Chanawong A, Lulitanond A, Wilailuckana C, Charoensri N, Sribenjalux P, Phumsrikaew W, Wonglakorn L, Kerdsin A, Chetchotisakd P. Rimrang B, et al. J Antimicrob Chemother. 2012 Nov;67(11):2626-30. doi: 10.1093/jac/dks267. Epub 2012 Jul 13. J Antimicrob Chemother. 2012. PMID: 22796889 - Evaluation of commercial phenotypic assays for the detection of IMP- or New Delhi metallo-β-lactamase-producing Enterobacteriaceae isolates in Japan.
Sakanashi D, Kawachi M, Uozumi Y, Nishio M, Hara Y, Suematsu H, Hagihara M, Nishiyama N, Asai N, Koizumi Y, Yamagishi Y, Mikamo H. Sakanashi D, et al. J Infect Chemother. 2017 Jul;23(7):474-480. doi: 10.1016/j.jiac.2017.04.003. Epub 2017 Apr 26. J Infect Chemother. 2017. PMID: 28456489 - Spread of carbapenemase NDM-1 producers: the situation in India and what may be proposed.
Khan AU, Nordmann P. Khan AU, et al. Scand J Infect Dis. 2012 Jul;44(7):531-5. doi: 10.3109/00365548.2012.669046. Epub 2012 Apr 12. Scand J Infect Dis. 2012. PMID: 22497308 Review.
Cited by
- Value of the modified Hodge test for detection of emerging carbapenemases in Enterobacteriaceae.
Girlich D, Poirel L, Nordmann P. Girlich D, et al. J Clin Microbiol. 2012 Feb;50(2):477-9. doi: 10.1128/JCM.05247-11. Epub 2011 Nov 23. J Clin Microbiol. 2012. PMID: 22116154 Free PMC article. - Identification of New Delhi metallo-β-lactamase 1 in Acinetobacter lwoffii of food animal origin.
Wang Y, Wu C, Zhang Q, Qi J, Liu H, Wang Y, He T, Ma L, Lai J, Shen Z, Liu Y, Shen J. Wang Y, et al. PLoS One. 2012;7(5):e37152. doi: 10.1371/journal.pone.0037152. Epub 2012 May 18. PLoS One. 2012. PMID: 22629360 Free PMC article. - Broad-Spectrum Cephalosporin-Resistant and/or Fluoroquinolone-Resistant Enterobacterales Associated with Canine and Feline Urogenital Infections.
Loncaric I, Misic D, Szostak MP, Künzel F, Schäfer-Somi S, Spergser J. Loncaric I, et al. Antibiotics (Basel). 2020 Jul 7;9(7):387. doi: 10.3390/antibiotics9070387. Antibiotics (Basel). 2020. PMID: 32645942 Free PMC article. - Resistance Detection and Transmission Risk Analysis of Pig-Derived Pathogenic Escherichia coli in East China.
Li X, Liu H, Cao S, Cheng P, Li F, Ishfaq M, Sun J, Zhang X. Li X, et al. Front Vet Sci. 2021 Apr 30;8:614651. doi: 10.3389/fvets.2021.614651. eCollection 2021. Front Vet Sci. 2021. PMID: 33996956 Free PMC article. - New Delhi Metallo-β-Lactamase-Mediated Carbapenem Resistance: Origin, Diagnosis, Treatment and Public Health Concern.
Wei WJ, Yang HF, Ye Y, Li JB. Wei WJ, et al. Chin Med J (Engl). 2015 Jul 20;128(14):1969-76. doi: 10.4103/0366-6999.160566. Chin Med J (Engl). 2015. PMID: 26168840 Free PMC article. Review.
References
- Center for Disease Control and Prevention 2009. Guidance for control of infections with carbapenem-resistant or carbapenemase-producing Enterobacteriaceae in acute care facilities. Morb. Mortal. Wkly. Rep. 58:256–266 - PubMed
- Centers for Disease Control and Prevention 2010. Detection of Enterobacteriaceae isolates carrying metallo-beta-lactamase—United States. Morb. Mortal. Wkly. Rep. 25:750 - PubMed
- Clinical and Laboratory Standards Institute 2010. Performance standards for antimicrobial susceptibility testing. CLSI M100-S20U. Update June 2010 Clinical and Laboratory Standards Institute, Wayne, PA
- Deshpande P., et al. 2010. New Delhi Metallo-beta lactamase (NDM-1) in Enterobacteriaceae: treatment options with carbapenems compromised. J. Assoc. Physicians India 58:147–149 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Miscellaneous