Analysis of the influence of PTPN22 gene polymorphisms in systemic sclerosis - PubMed (original) (raw)
Meta-Analysis
doi: 10.1136/ard.2010.130138. Epub 2010 Dec 3.
P Gourh, J Broen, C Simeon, V Fonollosa, N Ortego-Centeno, S Agarwal, M C Vonk, M Coenen, G Riemekasten, N Hunzelmann, R Hesselstrand, F K Tan, J D Reveille, S Assassi, F J García-Hernandez, P Carreira, M T Camps, A Fernandez-Nebro, P Garcia de la Peña, T Nearney, D Hilda, M A González-Gay, P Airo, L Beretta, R Scorza, A Herrick, J Worthington, A Pros, I Gómez-Gracia, L Trapiella, G Espinosa, I Castellvi, T Witte, F de Keyser, M Vanthuyne, M D Mayes, T R D J Radstake, F C Arnett, J Martin, B Rueda
Affiliations
- PMID: 21131644
- PMCID: PMC3170726
- DOI: 10.1136/ard.2010.130138
Meta-Analysis
Analysis of the influence of PTPN22 gene polymorphisms in systemic sclerosis
L M Diaz-Gallo et al. Ann Rheum Dis. 2011 Mar.
Erratum in
- Ann Rheum Dis. 2011 Aug;70(8):1520
Abstract
Objective: Two functional single nucleotide polymorphisms (SNP) in the PTPN22 gene (rs24746601 and rs33996649) have been associated with autoimmunity. The aim of this study was to investigate the role of the R263Q SNP for the first time and to re-evaluate the role of the R620W SNP in the genetic predisposition to systemic sclerosis (SSc) susceptibility and clinical phenotypes.
Methods: 3422 SSc patients (2020 with limited cutaneous SSc and 1208 with diffuse cutaneous SSc) and 3638 healthy controls of Caucasian ancestry from an initial case--control set of Spain and seven additional independent replication cohorts were included in our study. Both rs33996649 and rs2476601 PTPN22 polymorphisms were genotyped by TaqMan allelic discrimination assay. A meta-analysis was performed to test the overall effect of these PTPN22 polymorphisms in SSc.
Results: The meta-analysis revealed evidence of association of the rs2476601 T allele with SSc susceptibility (p(FDRcorrected)=0.03 pooled, OR 1.15, 95% CI 1.03 to 1.28). In addition, the rs2476601 T allele was significantly associated with anticentromere-positive status (p(FDRcorrected)=0.02 pooled, OR 1.22, 95% CI 1.05 to 1.42). Although the rs33996649 A allele was significantly associated with SSc in the Spanish population (p(FDRcorrected)=0.04, OR 0.58, 95% CI 0.36 to 0.92), this association was not confirmed in the meta-analysis (p=0.36 pooled, OR 0.89, 95% CI 0.72 to 1.1).
Conclusion: The study suggests that the PTPN22 R620W polymorphism influences SSc genetic susceptibility but the novel R263Q genetic variant does not. These data strengthen evidence that the R620W mutation is a common risk factor in autoimmune diseases.
Conflict of interest statement
Competing interests None.
Figures
Figure 1
(A) Forest plot for the meta-analysis of the PTPN22 R263Q (G788A; rs33996649) polymorphism in systemic sclerosis in eight Caucasian cohorts. (B) Forest plot of the PTPN22 R620W (C1858T; rs2476601) polymorphism and SSc in seven Caucasian cohorts and two previous studies.
Figure 2
(A) Forest plot for the meta-analysis of the PTPN22 the R620W (C1858T; rs2476601) polymorphism and limited cutaneous systemic sclerosis subtype, in seven Caucasian cohorts and previous studies. (B) Forest plot for the meta-analysis of the PTPN22 the R620W (C1858T; rs2476601) polymorphism and anticentromere antibody-positive autoantibody status of systemic sclerosis, in seven Caucasian cohorts and previous studies.
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