Altered mRNA expressions of sialyltransferases in human gastric cancer tissues - PubMed (original) (raw)

Altered mRNA expressions of sialyltransferases in human gastric cancer tissues

Li Jun et al. Med Oncol. 2012 Mar.

Abstract

Aberrant sialylation and altered sialyltransferase (ST) expressions are frequently found in many types of cancer. In this study, we examined the expressions of α 2,3-linked sialic acid residues and STs mRNA in human gastric cancer tissues. The relationship between the levels of α 2,3-linked sialic acid residues and the expression of STs mRNA was also analyzed. Maackia amurensis leukoagglutinin (MAL) specific for α 2,3-linked sialic acid was used for immunohistochemical staining. All gastric cancer tissues were positive for MAL staining, whereas adjacent normal tissues were negative. The extent of MAL staining was significantly correlated with the behavior of gastric cancer. The expression of STs mRNA was examined by real-time quantitative reverse transcription-polymerase chain reaction assay. The levels of ST3Gal IV and ST6GalI in gastric cancer tissues were significantly increased compared with those in adjacent normal tissues (P < 0.05), whereas the levels of ST3Gal I, ST3Gal III, and ST3Gal VI were not increased. Furthermore, the high expression of ST3Gal IV was closely related to the extent of MAL staining (P < 0.05) unlike that of ST6Gal I. These results indicated that gastric cancer tissues expressed high levels of α 2,3-linked sialic acid residues, ST3Gal IV, and ST6Gal I. The high expression of ST3Gal IV may contribute to the expression of α 2,3-linked sialic acid residues, which is associated with the malignant behavior of gastric cancer cells.

PubMed Disclaimer

Cited by

Modification of sialylation mediates the invasive properties and chemosensitivity of human hepatocellular carcinoma.
Zhao Y, Li Y, Ma H, Dong W, Zhou H, Song X, Zhang J, Jia L. Zhao Y, et al. Mol Cell Proteomics. 2014 Feb;13(2):520-36. doi: 10.1074/mcp.M113.034025. Epub 2013 Nov 19. Mol Cell Proteomics. 2014. PMID: 24255131 Free PMC article. Retracted.
Transmembrane mucins as novel therapeutic targets.
Constantinou PE, Danysh BP, Dharmaraj N, Carson DD. Constantinou PE, et al. Expert Rev Endocrinol Metab. 2011 Nov;6(6):835-848. doi: 10.1586/eem.11.70. Expert Rev Endocrinol Metab. 2011. PMID: 22201009 Free PMC article.
α2,6-linked sialic acids on N-glycans modulate the adhesion of hepatocarcinoma cells to lymph nodes.
Wang S, Chen X, Wei A, Yu X, Niang B, Zhang J. Wang S, et al. Tumour Biol. 2015 Feb;36(2):885-92. doi: 10.1007/s13277-014-2638-x. Epub 2014 Oct 11. Tumour Biol. 2015. PMID: 25304155
Reversal effect of ST6GAL 1 on multidrug resistance in human leukemia by regulating the PI3K/Akt pathway and the expression of P-gp and MRP1.
Ma H, Cheng L, Hao K, Li Y, Song X, Zhou H, Jia L. Ma H, et al. PLoS One. 2014 Jan 16;9(1):e85113. doi: 10.1371/journal.pone.0085113. eCollection 2014. PLoS One. 2014. PMID: 24454800 Free PMC article. Retracted.
Expression of ST3GAL4 leads to SLe(x) expression and induces c-Met activation and an invasive phenotype in gastric carcinoma cells.
Gomes C, Osório H, Pinto MT, Campos D, Oliveira MJ, Reis CA. Gomes C, et al. PLoS One. 2013 Jun 14;8(6):e66737. doi: 10.1371/journal.pone.0066737. Print 2013. PLoS One. 2013. PMID: 23799130 Free PMC article.

References

1. Cancer Res. 2005 Jun 1;65(11):4645-52 - PubMed
1. Gut. 2000 Mar;46(3):359-66 - PubMed
1. Int J Cancer. 2002 Jan 10;97(2):180-5 - PubMed
1. Biochem Biophys Res Commun. 2010 Jul 30;398(3):457-61 - PubMed
1. Glycobiology. 1998 Jul;8(7):707-17 - PubMed

Altered mRNA expressions of sialyltransferases in human gastric cancer tissues - PubMed (original) (raw)