Carbon monoxide oxidation by methanogenic bacteria - PubMed (original) (raw)

Carbon monoxide oxidation by methanogenic bacteria

L Daniels et al. J Bacteriol. 1977 Oct.

Abstract

Different species of methanogenic bacteria growing on CO(2) and H(2) were shown to remove CO added to the gas phase. Rates up to 0.2 mumol of CO depleted/min per 10 ml of culture containing approximately 7 mg of cells (wet weight) were observed. Methanobacterium thermoautotrophicum was selected for further study based on its ability to grow rapidly on a completely mineral medium. This species used CO as the sole energy source by disproportionating CO to CO(2) and CH(4) according to the following equation: 4CO + 2H(2)O --> 1CH(4) + 3CO(2). However, growth was slight, and the growth rate on CO was only 1% of that observed on H(2)/CO(2). Growth only occurred with CO concentrations in the gas phase of lower than 50%. Growth on CO agrees with the finding that cell-free extracts of M. thermoautotrophicum contained both an active factor 420 (F(420))-dependent hydrogenase (7.7 mumol/min per mg of protein at 35 degrees C) and a CO-dehydrogenating enzyme (0.2 mumol/min per mg of protein at 35 degrees C) that catalyzed the reduction of F(420) with CO. The properties of the CO-dehydrogenating enzyme are described. In addition to F(420), viologen dyes were effective electron acceptors for the enzyme. The apparent K(m) for CO was higher than 1 mM. The reaction rate increased with increasing pH and displayed an inflection point at pH 6.7. The temperature dependence of the reaction rate followed the Arrhenius equation with an activation energy (DeltaHdouble dagger) of 14.1 kcal/mol (59.0 kJ/mol). The CO dehydrogenase activity was reversibly inactivated by low concentrations of cyanide (2 muM) and was very sensitive to inactivation by oxygen. Carbon monoxide dehydrogenase of M. thermoautotrophicum exhibited several characteristic properties found for the enzyme of Clostridium pasteurianum but differed mainly in that the clostridial enzyme did not utilize F(420) as the electron acceptor.

PubMed Disclaimer

Cited by

Methanogens and the diversity of archaebacteria.
Jones WJ, Nagle DP Jr, Whitman WB. Jones WJ, et al. Microbiol Rev. 1987 Mar;51(1):135-77. doi: 10.1128/mr.51.1.135-177.1987. Microbiol Rev. 1987. PMID: 3104748 Free PMC article. Review. No abstract available.
Carbon monoxide oxidation by Clostridium thermoaceticum and Clostridium formicoaceticum.
Diekert GB, Thauer RK. Diekert GB, et al. J Bacteriol. 1978 Nov;136(2):597-606. doi: 10.1128/jb.136.2.597-606.1978. J Bacteriol. 1978. PMID: 711675 Free PMC article.
Acetate assimilation pathway of Methanosarcina barkeri.
Weimer PJ, Zeikus JG. Weimer PJ, et al. J Bacteriol. 1979 Jan;137(1):332-9. doi: 10.1128/jb.137.1.332-339.1979. J Bacteriol. 1979. PMID: 762016 Free PMC article.
Oxidoreductases and metal cofactors in the functioning of the earth.
Hay Mele B, Monticelli M, Leone S, Bastoni D, Barosa B, Cascone M, Migliaccio F, Montemagno F, Ricciardelli A, Tonietti L, Rotundi A, Cordone A, Giovannelli D. Hay Mele B, et al. Essays Biochem. 2023 Aug 11;67(4):653-670. doi: 10.1042/EBC20230012. Essays Biochem. 2023. PMID: 37503682 Free PMC article.
Presence of acetyl coenzyme A (CoA) carboxylase and propionyl-CoA carboxylase in autotrophic Crenarchaeota and indication for operation of a 3-hydroxypropionate cycle in autotrophic carbon fixation.
Menendez C, Bauer Z, Huber H, Gad'on N, Stetter KO, Fuchs G. Menendez C, et al. J Bacteriol. 1999 Feb;181(4):1088-98. doi: 10.1128/JB.181.4.1088-1098.1999. J Bacteriol. 1999. PMID: 9973333 Free PMC article.

References

1. Proc Natl Acad Sci U S A. 1976 Sep;73(9):3298-302 - PubMed
1. Antonie Van Leeuwenhoek. 1975;41(4):543-52 - PubMed
1. Biochim Biophys Acta. 1958 Oct;30(1):194-5 - PubMed
1. J Biol Chem. 1963 Aug;238:2882-6 - PubMed
1. J Biol Chem. 1951 Nov;193(1):265-75 - PubMed

Carbon monoxide oxidation by methanogenic bacteria - PubMed (original) (raw)