The fruitfly Drosophila melanogaster contains a novel charged adipokinetic-hormone-family peptide - PubMed (original) (raw)

The fruitfly Drosophila melanogaster contains a novel charged adipokinetic-hormone-family peptide

M H Schaffer et al. Biochem J. 1990.

Abstract

A member of the RPCH/AKH (red-pigment-concentrating hormone/adipokinetic hormone) family of arthropod neuropeptides was identified in the fruitfly Drosophila melanogaster, and its structure was determined by automated Edman degradation and m.s. using fast-atom-bombardment ionization and a tandem hybrid instrument capable of high sensitivity. The sequence of this peptide, which we call 'DAKH', is pGlu-Leu-Thr-Phe-Ser-Pro-Asp-Trp-NH2 (where pGlu is pyroglutamic acid and Trp-NH2 is tryptophan carboxyamide). H.p.l.c. analyses of extracts of the three body segments revealed that more than 80% of the peptide is contained in the thorax. Although DAKH is typical of family members in its general structure and distribution in the animal, it is unique in containing a residue which is charged under physiological conditions. The evolutionary significance of this change is considered.

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References

    1. Biochem Biophys Res Commun. 1985 Nov 27;133(1):337-42 - PubMed
    1. Rapid Commun Mass Spectrom. 1988 Sep;2(9):188-91 - PubMed
    1. Biochem Biophys Res Commun. 1986 Mar 13;135(2):622-8 - PubMed
    1. Annu Rev Biochem. 1986;55:773-99 - PubMed
    1. Proc Natl Acad Sci U S A. 1986 Sep;83(17):6233-7 - PubMed

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