Regulation of histidine and proline degradation enzymes by amino acid availability in Bacillus subtilis - PubMed (original) (raw)

Regulation of histidine and proline degradation enzymes by amino acid availability in Bacillus subtilis

M R Atkinson et al. J Bacteriol. 1990 Sep.

Abstract

The first enzymes of the histidine (hut) and proline degradative pathways, histidase and proline oxidase, could not be induced in Bacillus subtilis cells growing in glucose minimal medium containing a mixture of 16 amino acids. Addition of the 16-amino-acid mixture to induced wild-type cells growing in citrate minimal medium repressed histidase synthesis 25- to 250-fold and proline oxidase synthesis 16-fold. A strain containing a transcriptional fusion of the hut promoter to the beta-galactosidase gene was isolated from a library of Tn917-lacZ transpositions. Examination of histidase and beta-galactosidase expression in extracts of a hut-lacZ fusion strain grown in various media showed that induction, catabolite repression, and amino acid repression of the hut operon were mediated at the level of transcription. This result was confirmed by measurement of the steady-state level of hut RNA in cells grown in various media. Since amino acid repression was not defective in B. subtilis mutants deficient in nitrogen regulation of glutamine synthetase and catabolite repression, amino acid repression appears to be mediated by a system that functions independently of these regulatory systems.

PubMed Disclaimer

Cited by

Identification of genes and gene products whose expression is activated during nitrogen-limited growth in Bacillus subtilis.
Atkinson MR, Fisher SH. Atkinson MR, et al. J Bacteriol. 1991 Jan;173(1):23-7. doi: 10.1128/jb.173.1.23-27.1991. J Bacteriol. 1991. PMID: 1670935 Free PMC article.
Heavy involvement of stringent transcription control depending on the adenine or guanine species of the transcription initiation site in glucose and pyruvate metabolism in Bacillus subtilis.
Tojo S, Kumamoto K, Hirooka K, Fujita Y. Tojo S, et al. J Bacteriol. 2010 Mar;192(6):1573-85. doi: 10.1128/JB.01394-09. Epub 2010 Jan 15. J Bacteriol. 2010. PMID: 20081037 Free PMC article.
Dual regulation of the Bacillus subtilis regulon comprising the lmrAB and yxaGH operons and yxaF gene by two transcriptional repressors, LmrA and YxaF, in response to flavonoids.
Hirooka K, Kunikane S, Matsuoka H, Yoshida K, Kumamoto K, Tojo S, Fujita Y. Hirooka K, et al. J Bacteriol. 2007 Jul;189(14):5170-82. doi: 10.1128/JB.00079-07. Epub 2007 May 4. J Bacteriol. 2007. PMID: 17483215 Free PMC article.
TnrA, a transcription factor required for global nitrogen regulation in Bacillus subtilis.
Wray LV Jr, Ferson AE, Rohrer K, Fisher SH. Wray LV Jr, et al. Proc Natl Acad Sci U S A. 1996 Aug 20;93(17):8841-5. doi: 10.1073/pnas.93.17.8841. Proc Natl Acad Sci U S A. 1996. PMID: 8799114 Free PMC article.
Indirect repression by Bacillus subtilis CodY via displacement of the activator of the proline utilization operon.
Belitsky BR. Belitsky BR. J Mol Biol. 2011 Oct 21;413(2):321-36. doi: 10.1016/j.jmb.2011.08.003. Epub 2011 Aug 5. J Mol Biol. 2011. PMID: 21840319 Free PMC article.

References

1. Plasmid. 1984 Sep;12(2):119-38 - PubMed
1. J Bacteriol. 1984 Apr;158(1):379-82 - PubMed
1. J Bacteriol. 1985 Nov;164(2):938-40 - PubMed
1. J Bacteriol. 1986 Jul;167(1):35-43 - PubMed
1. J Bacteriol. 1988 Jul;170(7):3199-205 - PubMed

Regulation of histidine and proline degradation enzymes by amino acid availability in Bacillus subtilis - PubMed (original) (raw)