Real time, high resolution video imaging of apoptosis in single cells with a polymeric nanoprobe - PubMed (original) (raw)

. 2011 Feb 16;22(2):125-31.

doi: 10.1021/bc1004119. Epub 2011 Jan 10.

Ki Young Choi, Hyunjin Chung, Ju Hee Ryu, Aeju Lee, Heebeom Koo, In-Chan Youn, Jae Hyung Park, In-San Kim, Sang Yoon Kim, Xiaoyuan Chen, Seo Young Jeong, Ick Chan Kwon, Kwangmeyung Kim, Kuiwon Choi

Affiliations

Real time, high resolution video imaging of apoptosis in single cells with a polymeric nanoprobe

Seulki Lee et al. Bioconjug Chem. 2011.

Abstract

We report a new apoptosis nanoprobe (Apo-NP) designed on the basis of a polymer nanoparticle platform. This simple one-step technique is capable of boosting fluorescence signals upon apoptosis in living cells, enabling real-time imaging of apoptosis in single cells and in vivo. The Apo-NP efficiently delivers chemically labeled, dual-quenched caspase-3-sensitive fluorogenic peptides into cells, allowing caspase-3-dependent strong fluorescence amplification to be imaged in apoptotic cells in real-time and at high resolution. The design platform of the Apo-NP is flexible and can be fine-tuned for a wide array of applications such as identification of caspase-related apoptosis in pathologies and for monitoring therapeutic efficacy of apoptotic drugs in cancer treatment.

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