Proteome half-life dynamics in living human cells - PubMed (original) (raw)

. 2011 Feb 11;331(6018):764-8.

doi: 10.1126/science.1199784. Epub 2011 Jan 13.

Affiliations

• PMID: 21233346
• DOI: 10.1126/science.1199784

Proteome half-life dynamics in living human cells

Eran Eden et al. Science. 2011.

Abstract

Cells remove proteins by two processes: degradation and dilution due to cell growth. The balance between these basic processes is poorly understood. We addressed this by developing an accurate and noninvasive method for measuring protein half-lives, called "bleach-chase," that is applicable to fluorescently tagged proteins. Assaying 100 proteins in living human cancer cells showed half-lives that ranged between 45 minutes and 22.5 hours. A variety of stresses that stop cell division showed the same general effect: Long-lived proteins became longer-lived, whereas short-lived proteins remained largely unaffected. This effect is due to the relative strengths of degradation and dilution and suggests a mechanism for differential killing of rapidly growing cells by growth-arresting drugs. This approach opens a way to understand proteome half-life dynamics in living cells.

PubMed Disclaimer

Comment in

• Cell biology. The lives of proteins.
  Plotkin JB. Plotkin JB. Science. 2011 Feb 11;331(6018):683-4. doi: 10.1126/science.1202010. Science. 2011. PMID: 21310990 No abstract available.
• A day in the half-life of a protein.
  Doerr A. Doerr A. Nat Methods. 2011 Mar;8(3):201. doi: 10.1038/nmeth0311-201. Nat Methods. 2011. PMID: 21473019

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Atypon
  • Ovid Technologies, Inc.

• Other Literature Sources

  • H1 Connect
  • The Lens - Patent Citations