The mtr locus is a two-gene operon required for transcription attenuation in the trp operon of Bacillus subtilis - PubMed (original) (raw)

Comparative Study

The mtr locus is a two-gene operon required for transcription attenuation in the trp operon of Bacillus subtilis

P Gollnick et al. Proc Natl Acad Sci U S A. 1990 Nov.

Abstract

We have cloned and characterized the mtr operon of Bacillus subtilis. This operon encodes a presumed RNA-binding regulatory protein that is required for attenuation control of the trp operon. We have shown that the mtr operon consists of two structural genes, mtrA and mtrB, predicted to encode 22-kDa and 8-kDa polypeptides, respectively. MtrB shows homology with RegA, an RNA-binding regulatory protein of bacteriophage T4. The lesions in several mtr mutants were localized to mtrB or the putative mtr promoter. Several mtrB alleles were dominant to mtr+, suggesting that the regulatory factor is a multimeric protein. The in vivo action of the mtrA and mtrB gene products was analyzed in an E. coli strain containing a trpE-lacZ gene fusion under control of the B. subtilis trp promoter/attenuator region. Both MtrA and MtrB were necessary for regulation of beta-galactosidase production.

PubMed Disclaimer

Similar articles

• The mtrAB operon of Bacillus subtilis encodes GTP cyclohydrolase I (MtrA), an enzyme involved in folic acid biosynthesis, and MtrB, a regulator of tryptophan biosynthesis.
  Babitzke P, Gollnick P, Yanofsky C. Babitzke P, et al. J Bacteriol. 1992 Apr;174(7):2059-64. doi: 10.1128/jb.174.7.2059-2064.1992. J Bacteriol. 1992. PMID: 1551827 Free PMC article.
• cis-acting sites in the transcript of the Bacillus subtilis trp operon regulate expression of the operon.
  Kuroda MI, Henner D, Yanofsky C. Kuroda MI, et al. J Bacteriol. 1988 Jul;170(7):3080-8. doi: 10.1128/jb.170.7.3080-3088.1988. J Bacteriol. 1988. PMID: 3133360 Free PMC article.
• MtrB from Bacillus subtilis binds specifically to trp leader RNA in a tryptophan-dependent manner.
  Otridge J, Gollnick P. Otridge J, et al. Proc Natl Acad Sci U S A. 1993 Jan 1;90(1):128-32. doi: 10.1073/pnas.90.1.128. Proc Natl Acad Sci U S A. 1993. PMID: 8419914 Free PMC article.
• Regulation of the Bacillus subtilis trp operon by an RNA-binding protein.
  Gollnick P. Gollnick P. Mol Microbiol. 1994 Mar;11(6):991-7. doi: 10.1111/j.1365-2958.1994.tb00377.x. Mol Microbiol. 1994. PMID: 8022289 Review.
• The different roles of tryptophan transfer RNA in regulating trp operon expression in E. coli versus B. subtilis.
  Yanofsky C. Yanofsky C. Trends Genet. 2004 Aug;20(8):367-74. doi: 10.1016/j.tig.2004.06.007. Trends Genet. 2004. PMID: 15262409 Review.

Cited by

• Molecular cloning, nucleotide sequence, and characterization of the Bacillus subtilis gene encoding the DNA-binding protein HBsu.
  Micka B, Groch N, Heinemann U, Marahiel MA. Micka B, et al. J Bacteriol. 1991 May;173(10):3191-8. doi: 10.1128/jb.173.10.3191-3198.1991. J Bacteriol. 1991. PMID: 1902464 Free PMC article.
• Translation of trpG in Bacillus subtilis is regulated by the trp RNA-binding attenuation protein (TRAP).
  Yang M, de Saizieu A, van Loon AP, Gollnick P. Yang M, et al. J Bacteriol. 1995 Aug;177(15):4272-8. doi: 10.1128/jb.177.15.4272-4278.1995. J Bacteriol. 1995. PMID: 7543470 Free PMC article.
• The TRAP-like SplA protein is a trans-acting negative regulator of spore photoproduct lyase synthesis during Bacillus subtilis sporulation.
  Fajardo-Cavazos P, Nicholson WL. Fajardo-Cavazos P, et al. J Bacteriol. 2000 Jan;182(2):555-60. doi: 10.1128/JB.182.2.555-560.2000. J Bacteriol. 2000. PMID: 10629212 Free PMC article.
• trp RNA-binding attenuation protein-5' stem-loop RNA interaction is required for proper transcription attenuation control of the Bacillus subtilis trpEDCFBA operon.
  Du H, Yakhnin AV, Dharmaraj S, Babitzke P. Du H, et al. J Bacteriol. 2000 Apr;182(7):1819-27. doi: 10.1128/JB.182.7.1819-1827.2000. J Bacteriol. 2000. PMID: 10714985 Free PMC article.
• Enzymic characterization of Bacillus subtilis GTP cyclohydrolase I. Evidence for a chemical dephosphorylation of dihydroneopterin triphosphate.
  De Saizieu A, Vankan P, van Loon AP. De Saizieu A, et al. Biochem J. 1995 Mar 1;306 ( Pt 2)(Pt 2):371-7. Biochem J. 1995. PMID: 7887891 Free PMC article.

References

1. 1. Proc Natl Acad Sci U S A. 1972 Aug;69(8):2110-4 - PubMed
2. 1. J Biol Chem. 1972 Mar 10;247(5):1587-96 - PubMed
3. 1. Methods Enzymol. 1979;68:342-57 - PubMed
4. 1. Nucleic Acids Res. 1984 Jan 11;12(1 Pt 1):387-95 - PubMed
5. 1. Proc Natl Acad Sci U S A. 1984 Jan;81(2):439-43 - PubMed

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Atypon
  • Europe PubMed Central
  • PubMed Central

• Other Literature Sources

  • The Lens - Patent Citations Database

• Molecular Biology Databases

  • BioCyc