Recovery of transforming EBV from non-producer cells after superinfection with non-transforming P3HR-1 EBV - PubMed (original) (raw)
Recovery of transforming EBV from non-producer cells after superinfection with non-transforming P3HR-1 EBV
K O Fresen et al. Int J Cancer. 1978.
Abstract
Cells of the Raji and NC37 lines can be induced by chemical inducers, such as BrdUrd and IdUrd, or the tumor-promoter TPA to EA-expression only, but do not reveal any VCA synthesis. After superinfection by nontransforming P3HR-1 EBV, however, a varying percentage of the cell population shows VCA synthesis and releases infectious viral particles. The recovered virus differs biologically from P3HR-1 EBV since it transforms human umbilical cord blood lymphocytes into EBNA-positive lymphoblastoid cell lines. Cells of these established lines are susceptible to renewed infection by P3HR-1 EBV which results in EA induction and VCA synthesis. Only cells of one line, NC37-R1, spontaneously produce VCA and EBV particles, which reveal transforming properties and do not induce EA upon superinfection of Raji cells. Infection of P3HR-1 EBV-converted BJA-B cells also leads to EA and VCA induction and the release of viral particles. In contrast to particles recovered from Raji and NC37 cells, no transforming activity was detectable in these virus preparations. According to these data, we propose that viral genomes persisting within Raji and NC37 cells are defective and become complemented by the superinfecting P3HR-1 virus.
Similar articles
- NC37-R1 Epstein-Barr virus (EBV): a possible recombinant between intracellular NC37 viral DNA and superinfecting P3HR-1 EBV.
Fresen KO, Cho MS, Gissmann L, zur Hausen H. Fresen KO, et al. Intervirology. 1980;12(6):303-10. doi: 10.1159/000149089. Intervirology. 1980. PMID: 6244234 - Productive Epstein-Barr viral infection of the human lymphoblastoid cell line 6410 with release of early antigen inducing and transforming virus.
Traul KA, Stephens R, Gerber P, Peterson WD. Traul KA, et al. Int J Cancer. 1977 Aug 15;20(2):247-55. doi: 10.1002/ijc.2910200213. Int J Cancer. 1977. PMID: 197026
Cited by
- Epstein-Barr virus stimulates torque teno virus replication: a possible relationship to multiple sclerosis.
Borkosky SS, Whitley C, Kopp-Schneider A, zur Hausen H, de Villiers EM. Borkosky SS, et al. PLoS One. 2012;7(2):e32160. doi: 10.1371/journal.pone.0032160. Epub 2012 Feb 22. PLoS One. 2012. PMID: 22384166 Free PMC article. - Molecular virology of Epstein-Barr virus.
Bornkamm GW, Hammerschmidt W. Bornkamm GW, et al. Philos Trans R Soc Lond B Biol Sci. 2001 Apr 29;356(1408):437-59. doi: 10.1098/rstb.2000.0781. Philos Trans R Soc Lond B Biol Sci. 2001. PMID: 11313004 Free PMC article. Review. - Identification of a naturally occurring recombinant Epstein-Barr virus isolate from New Guinea that encodes both type 1 and type 2 nuclear antigen sequences.
Burrows JM, Khanna R, Sculley TB, Alpers MP, Moss DJ, Burrows SR. Burrows JM, et al. J Virol. 1996 Jul;70(7):4829-33. doi: 10.1128/JVI.70.7.4829-4833.1996. J Virol. 1996. PMID: 8676517 Free PMC article. - Structure of defective DNA molecules in Epstein-Barr virus preparations from P3HR-1 cells.
Cho MS, Bornkamm GW, zur Hausen H. Cho MS, et al. J Virol. 1984 Jul;51(1):199-207. doi: 10.1128/JVI.51.1.199-207.1984. J Virol. 1984. PMID: 6328039 Free PMC article. - Identification of a rare Epstein-Barr virus variant that enhances early antigen expression in Raji cells.
Rabson M, Heston L, Miller G. Rabson M, et al. Proc Natl Acad Sci U S A. 1983 May;80(9):2762-6. doi: 10.1073/pnas.80.9.2762. Proc Natl Acad Sci U S A. 1983. PMID: 6302703 Free PMC article.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources