Chemoproteomics profiling of HDAC inhibitors reveals selective targeting of HDAC complexes - PubMed (original) (raw)

doi: 10.1038/nbt.1759. Epub 2011 Jan 23.

Carsten Hopf, Mikhail M Savitski, Antje Dittmann, Paola Grandi, Anne-Marie Michon, Judith Schlegl, Yann Abraham, Isabelle Becher, Giovanna Bergamini, Markus Boesche, Manja Delling, Birgit Dümpelfeld, Dirk Eberhard, Carola Huthmacher, Toby Mathieson, Daniel Poeckel, Valérie Reader, Katja Strunk, Gavain Sweetman, Ulrich Kruse, Gitte Neubauer, Nigel G Ramsden, Gerard Drewes

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Chemoproteomics profiling of HDAC inhibitors reveals selective targeting of HDAC complexes

Marcus Bantscheff et al. Nat Biotechnol. 2011 Mar.

Abstract

The development of selective histone deacetylase (HDAC) inhibitors with anti-cancer and anti-inflammatory properties remains challenging in large part owing to the difficulty of probing the interaction of small molecules with megadalton protein complexes. A combination of affinity capture and quantitative mass spectrometry revealed the selectivity with which 16 HDAC inhibitors target multiple HDAC complexes scaffolded by ELM-SANT domain subunits, including a novel mitotic deacetylase complex (MiDAC). Inhibitors clustered according to their target profiles with stronger binding of aminobenzamides to the HDAC NCoR complex than to the HDAC Sin3 complex. We identified several non-HDAC targets for hydroxamate inhibitors. HDAC inhibitors with distinct profiles have correspondingly different effects on downstream targets. We also identified the anti-inflammatory drug bufexamac as a class IIb (HDAC6, HDAC10) HDAC inhibitor. Our approach enables the discovery of novel targets and inhibitors and suggests that the selectivity of HDAC inhibitors should be evaluated in the context of HDAC complexes and not purified catalytic subunits.

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References

    1. Cell. 2007 Feb 23;128(4):693-705 - PubMed
    1. Curr Opin Genet Dev. 2008 Oct;18(5):404-10 - PubMed
    1. Cell. 2009 Dec 11;139(6):1029-31 - PubMed
    1. Nat Rev Drug Discov. 2006 Sep;5(9):769-84 - PubMed
    1. J Proteome Res. 2009 Oct;8(10):4743-52 - PubMed

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