Intrathymic selection of murine TCR alpha beta+CD4-CD8- thymocytes - PubMed (original) (raw)
Comparative Study
Intrathymic selection of murine TCR alpha beta+CD4-CD8- thymocytes
M Egerton et al. Int Immunol. 1990.
Abstract
The CD4-CD8- thymocyte population contains the precursors of all other thymocytes. However, it also contains a significant proportion of cells which express surface TCR alpha beta, and have little or no precursor activity. Like peripheral T cells, but unlike most other thymocytes, these TCR alpha beta+CD4-CD8- thymocytes do not express heat stable antigen. Both the origin and developmental status of these cells are unclear, and are the subject of this report. We have measured the proportion of V beta 8.1+ cells amongst TCR+HSA-CD4-CD8- thymocytes in MIs-1a versus MIs-1b mice, in order to determine whether they have undergone negative selection. The proportions were similar in both strains, in contrast to mature T cells, indicating that neither they nor their precursors had undergone clonal deletion. We also measured the accumulation of these cells over the early life of the animal and found that it was extremely slow. Our data also show that although TCR-V beta 8.1+ cells are reactive to MIs-1a in association with MHC class II, most mature TCR-V beta 8.1+ cells in MIs-1b mice are CD8+, suggesting an additional reactivity with MHC class I. We raise the possibility that TCR-V beta 8.1+CD4-CD8- thymocytes are derived from TCR-V beta 8.1+CD4+CD8+ thymocytes, and that the reactivity of TCR-V beta 8.1 with both MHC classes I and II has resulted in the down-regulation of both CD4 and CD8.
Similar articles
- CD4-CD8- thymocytes that express the T cell receptor may have previously expressed CD8.
Wu L, Pearse M, Egerton M, Petrie H, Scollay R. Wu L, et al. Int Immunol. 1990;2(1):51-6. doi: 10.1093/intimm/2.1.51. Int Immunol. 1990. PMID: 2150920 - In vivo modulation of the distribution of thymocyte subsets: effects of estrogen on the expression of different T cell receptor V beta gene families in CD4-, CD8- thymocytes.
Screpanti I, Meco D, Morrone S, Gulino A, Mathieson BJ, Frati L. Screpanti I, et al. Cell Immunol. 1991 May;134(2):414-26. doi: 10.1016/0008-8749(91)90314-2. Cell Immunol. 1991. PMID: 1708703 - Developmental status and reconstitution potential of subpopulations of murine thymocytes.
Scollay R, Wilson A, D'Amico A, Kelly K, Egerton M, Pearse M, Wu L, Shortman K. Scollay R, et al. Immunol Rev. 1988 Aug;104:81-120. doi: 10.1111/j.1600-065x.1988.tb00760.x. Immunol Rev. 1988. PMID: 3049319 Review. - Developmental regulation of alpha beta T cell antigen receptor assembly in immature CD4+CD8+ thymocytes.
Kearse KP, Roberts JP, Wiest DL, Singer A. Kearse KP, et al. Bioessays. 1995 Dec;17(12):1049-54. doi: 10.1002/bies.950171209. Bioessays. 1995. PMID: 8634066 Review.
Cited by
- Double Negative T Regulatory Cells: An Emerging Paradigm Shift in Reproductive Immune Tolerance?
Bafor EE, Valencia JC, Young HA. Bafor EE, et al. Front Immunol. 2022 Jul 1;13:886645. doi: 10.3389/fimmu.2022.886645. eCollection 2022. Front Immunol. 2022. PMID: 35844500 Free PMC article. Review. - TCRαβ+ CD4-/CD8- "double negative" T cells in health and disease-implications for the kidney.
Newman-Rivera AM, Kurzhagen JT, Rabb H. Newman-Rivera AM, et al. Kidney Int. 2022 Jul;102(1):25-37. doi: 10.1016/j.kint.2022.02.035. Epub 2022 Apr 9. Kidney Int. 2022. PMID: 35413379 Free PMC article. Review. - Double negative regulatory T cells in transplantation and autoimmunity: recent progress and future directions.
Juvet SC, Zhang L. Juvet SC, et al. J Mol Cell Biol. 2012 Feb;4(1):48-58. doi: 10.1093/jmcb/mjr043. J Mol Cell Biol. 2012. PMID: 22294241 Free PMC article. Review. - Quick recovery in the generation of self-reactive CD4low natural killer (NK) T cells by an alternative intrathymic pathway when restored from acute thymic atrophy.
Maruyama S, Tsukahara A, Suzuki S, Tada T, Minagawa M, Watanabe H, Hatakeyama K, Abo T. Maruyama S, et al. Clin Exp Immunol. 1999 Sep;117(3):587-95. doi: 10.1046/j.1365-2249.1999.00988.x. Clin Exp Immunol. 1999. PMID: 10469067 Free PMC article. - Autoreactive CD4- CD8- alpha beta T cells to vaccinate adjuvant arthritis.
Haque MA, Kimoto M, Inada S, Tokunaga O, Kohashi O. Haque MA, et al. Immunology. 1998 Aug;94(4):536-42. doi: 10.1046/j.1365-2567.1998.00543.x. Immunology. 1998. PMID: 9767442 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Research Materials