DNA sequence analysis of the imp UV protection and mutation operon of the plasmid TP110: identification of a third gene - PubMed (original) (raw)
DNA sequence analysis of the imp UV protection and mutation operon of the plasmid TP110: identification of a third gene
D Lodwick et al. Nucleic Acids Res. 1990.
Free PMC article
Abstract
The sequence of the imp operon of the plasmid TP110 (which belongs to the Incl1 incompatibility group) has been determined, and is shown to contain three open reading frames. This operon, involved in UV protection and mutation, is functionally analogous to the umuDC operon of E. coli and the mucAB operon of the plasmid pKM101, which belongs to the quite unrelated IncN incompatibility group. The umu and muc operons however contain only two open reading frames, coding for proteins of approximately 16kD and 46kD. The high degree of homology between the two 16kD proteins (UmuD and MucA) and between the two 46kD proteins (UmuC and MucB) clearly shows their relatedness. This is shown also to extend to the imp gene products, with ImpA sharing homology with UmuD and MucA, and ImpB sharing homology with UmuC and MucB. However, the two imp genes are preceded in the operon by a third gene, impC, which encodes a small protein of 9.5kD and which has no equivalent in the umu and muc operons.
Similar articles
- umuDC and mucAB operons whose products are required for UV light- and chemical-induced mutagenesis: UmuD, MucA, and LexA proteins share homology.
Perry KL, Elledge SJ, Mitchell BB, Marsh L, Walker GC. Perry KL, et al. Proc Natl Acad Sci U S A. 1985 Jul;82(13):4331-5. doi: 10.1073/pnas.82.13.4331. Proc Natl Acad Sci U S A. 1985. PMID: 2989816 Free PMC article. - umuDC and mucAB operons whose products are required for UV light- and chemical-induced mutagenesis: UmuD, MucA, and LexA proteins share homology. 1985.
Perry KL, Elledge SJ, Mitchell BB, Marsh L, Walker GC. Perry KL, et al. DNA Repair (Amst). 2005 Aug 15;4(9):1054-8. DNA Repair (Amst). 2005. PMID: 16196126 No abstract available. - The virulence plasmid-encoded impCAB operon enhances survival and induced mutagenesis in Shigella flexneri after exposure to UV radiation.
Runyen-Janecky LJ, Hong M, Payne SM. Runyen-Janecky LJ, et al. Infect Immun. 1999 Mar;67(3):1415-23. doi: 10.1128/IAI.67.3.1415-1423.1999. Infect Immun. 1999. PMID: 10024589 Free PMC article. - Plasmid genes affecting DNA repair and mutation.
Strike P, Lodwick D. Strike P, et al. J Cell Sci Suppl. 1987;6:303-21. doi: 10.1242/jcs.1984.supplement_6.20. J Cell Sci Suppl. 1987. PMID: 3308922 Review. - Mutagenesis induced by bacterial UmuDC proteins and their plasmid homologues.
Woodgate R, Sedgwick SG. Woodgate R, et al. Mol Microbiol. 1992 Aug;6(16):2213-8. doi: 10.1111/j.1365-2958.1992.tb01397.x. Mol Microbiol. 1992. PMID: 1406263 Review.
Cited by
- Diverse anti-defence systems are encoded in the leading region of plasmids.
Samuel B, Mittelman K, Croitoru SY, Ben Haim M, Burstein D. Samuel B, et al. Nature. 2024 Oct 9. doi: 10.1038/s41586-024-07994-w. Online ahead of print. Nature. 2024. PMID: 39385022 - Genetic basis of I-complex plasmid stability and conjugation.
Lian ZJ, Phan MD, Hancock SJ, Nhu NTK, Paterson DL, Schembri MA. Lian ZJ, et al. PLoS Genet. 2023 Jun 22;19(6):e1010773. doi: 10.1371/journal.pgen.1010773. eCollection 2023 Jun. PLoS Genet. 2023. PMID: 37347771 Free PMC article. - Identification and Characterisation of pST1023 A Mosaic, Multidrug-Resistant and Mobilisable IncR Plasmid.
Calia C, Oliva M, Ferrara M, Minervini CF, Scrascia M, Monno R, Mulè G, Cumbo C, Marzella A, Pazzani C. Calia C, et al. Microorganisms. 2022 Aug 8;10(8):1592. doi: 10.3390/microorganisms10081592. Microorganisms. 2022. PMID: 36014010 Free PMC article. - Incompatibility Group I1 (IncI1) Plasmids: Their Genetics, Biology, and Public Health Relevance.
Foley SL, Kaldhone PR, Ricke SC, Han J. Foley SL, et al. Microbiol Mol Biol Rev. 2021 Apr 28;85(2):e00031-20. doi: 10.1128/MMBR.00031-20. Print 2021 May 19. Microbiol Mol Biol Rev. 2021. PMID: 33910982 Free PMC article. Review. - Genetic characterization of three qnrS1-harbouring multidrug-resistance plasmids and qnrS1-containing transposons circulating in Ho Chi Minh City, Vietnam.
Le V, Nhu NTK, Cerdeno-Tarraga A, Campbell JI, Tuyen HT, Nhu TDH, Tam PTT, Schultsz C, Thwaites G, Thomson NR, Baker S. Le V, et al. J Med Microbiol. 2015 Aug;64(8):869-878. doi: 10.1099/jmm.0.000100. J Med Microbiol. 2015. PMID: 26272054 Free PMC article.
References
- Proc Natl Acad Sci U S A. 1975 Mar;72(3):979-83 - PubMed
- Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 - PubMed
- Gene. 1982 Oct;19(3):269-76 - PubMed
- Proc Natl Acad Sci U S A. 1983 Jul;80(13):3963-5 - PubMed
- Microbiol Rev. 1984 Mar;48(1):60-93 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources