Sequencing transcriptomes in toto - PubMed (original) (raw)

Review

doi: 10.1039/c0ib00062k. Epub 2011 Feb 4.

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Review

Sequencing transcriptomes in toto

Karin S Kassahn et al. Integr Biol (Camb). 2011 May.

Abstract

The development of next-generation sequencing technologies has enabled the transcriptome to be measured and characterized at a level which was previously unattainable. Shot gun sequencing of RNAs, or RNA-Seq as it is known, is providing the means to simultaneously survey locus activity, transcript-specific expression, sequence content of transcripts and transcriptome discovery. This article discusses the current state of RNA-Seq, its potential for redefining transcriptomics and some of the challenges associated with this revolutionary technology.

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