Sequencing transcriptomes in toto - PubMed (original) (raw)
Review
doi: 10.1039/c0ib00062k. Epub 2011 Feb 4.
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- PMID: 21298135
- DOI: 10.1039/c0ib00062k
Review
Sequencing transcriptomes in toto
Karin S Kassahn et al. Integr Biol (Camb). 2011 May.
Abstract
The development of next-generation sequencing technologies has enabled the transcriptome to be measured and characterized at a level which was previously unattainable. Shot gun sequencing of RNAs, or RNA-Seq as it is known, is providing the means to simultaneously survey locus activity, transcript-specific expression, sequence content of transcripts and transcriptome discovery. This article discusses the current state of RNA-Seq, its potential for redefining transcriptomics and some of the challenges associated with this revolutionary technology.
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