miR-27b*, an oxidative stress-responsive microRNA modulates nuclear factor-kB pathway in RAW 264.7 cells - PubMed (original) (raw)

. 2011 Jun;352(1-2):181-8.

doi: 10.1007/s11010-011-0752-2. Epub 2011 Feb 25.

Affiliations

Free article

miR-27b*, an oxidative stress-responsive microRNA modulates nuclear factor-kB pathway in RAW 264.7 cells

Sivasubramani Thulasingam et al. Mol Cell Biochem. 2011 Jun.

Free article

Abstract

Reactive oxygen species (ROS) produced in macrophages is critical for microbial killing, but they also take part in inflammation and antigen presentation functions. MicroRNAs (miRNAs) are endogenous regulators of gene expression, and they can control immune responses. To dissect the complex nature of ROS-mediated effects in macrophages, we sought to characterize miRNAs that are responsive to oxidative stress-induced with hydrogen peroxide (H(2)O(2)) in the mouse macrophage cell line, RAW 264.7. We have identified a set of unique miRNAs that are differentially expressed in response to H(2)O(2). These include miR-27a*, miR-27b*, miR-29b*, miR-24-2*, and miR-21*, all of which were downregulated except for miR-21*. By using luciferase reporter vector containing nuclear factor-kB (NF-kB) response elements, we demonstrate that overexpression of miR-27b* suppresses lipopolysaccharide-induced activation of NF-kB in RAW 264.7 cells. Our data suggest that macrophage functions can be regulated by oxidative stress-responsive miRNAs by modulating the NF-kB pathway.

PubMed Disclaimer

Similar articles

Cited by

References

    1. Cell. 2007 Oct 5;131(1):146-59 - PubMed
    1. Apoptosis. 2005 May;10(3):545-56 - PubMed
    1. Clin Microbiol Rev. 2009 Apr;22(2):240-73, Table of Contents - PubMed
    1. Nat Immunol. 2008 Apr;9(4):405-14 - PubMed
    1. Bioinformatics. 2005 May 1;21(9):2067-75 - PubMed

Publication types

MeSH terms

Substances

LinkOut - more resources