Imaging real-time gene expression in living yeast - PubMed (original) (raw)
Imaging real-time gene expression in living yeast
Daniel Zenklusen et al. CSH Protoc. 2007.
Abstract
INTRODUCTIONThis protocol describes the application of the MS2 system to the yeast Saccharomyces cerevisiae. ASH1 mRNA tagged with six MS2 repeats (6MBSs) is used to follow the localization of the ASH1 mRNA particles to the bud tip of a haploid yeast cell. W303 yeast cells transformed with pG14-MS2-GFP and pGAL-lacZ-MS2-ASH1 are grown on select medium lacking tryptophan and leucine. RNA expression is induced by the addition of galactose, and a time-lapse movie is then acquired.
Figures
Figure 1
Localization of the daughter-cell-specific ASH1 mRNA to the bud tip of a daughter cell in S. cerevisiae. Bar, 5 μm. (Reprinted from Bertrand et al. 1998, with permission from Elsevier © 1998.)
Similar articles
- Localization and anchoring of mRNA in budding yeast.
Beach DL, Salmon ED, Bloom K. Beach DL, et al. Curr Biol. 1999 Jun 3;9(11):569-78. doi: 10.1016/s0960-9822(99)80260-7. Curr Biol. 1999. PMID: 10359695 - Localization of ASH1 mRNA particles in living yeast.
Bertrand E, Chartrand P, Schaefer M, Shenoy SM, Singer RH, Long RM. Bertrand E, et al. Mol Cell. 1998 Oct;2(4):437-45. doi: 10.1016/s1097-2765(00)80143-4. Mol Cell. 1998. PMID: 9809065 - Structural elements required for the localization of ASH1 mRNA and of a green fluorescent protein reporter particle in vivo.
Chartrand P, Meng XH, Singer RH, Long RM. Chartrand P, et al. Curr Biol. 1999 Mar 25;9(6):333-6. doi: 10.1016/s0960-9822(99)80144-4. Curr Biol. 1999. PMID: 10209102 - RNA asymmetric distribution and daughter/mother differentiation in yeast.
Darzacq X, Powrie E, Gu W, Singer RH, Zenklusen D. Darzacq X, et al. Curr Opin Microbiol. 2003 Dec;6(6):614-20. doi: 10.1016/j.mib.2003.10.005. Curr Opin Microbiol. 2003. PMID: 14662358 Free PMC article. Review. - RNA localization in yeast: moving towards a mechanism.
Gonsalvez GB, Urbinati CR, Long RM. Gonsalvez GB, et al. Biol Cell. 2005 Jan;97(1):75-86. doi: 10.1042/BC20040066. Biol Cell. 2005. PMID: 15601259 Review.
Cited by
- Dynamic imaging demonstrates that pulsed electromagnetic fields (PEMF) suppress IL-6 transcription in bovine nucleus pulposus cells.
Tang X, Alliston T, Coughlin D, Miller S, Zhang N, Waldorff EI, Ryaby JT, Lotz JC. Tang X, et al. J Orthop Res. 2018 Feb;36(2):778-787. doi: 10.1002/jor.23713. Epub 2017 Oct 17. J Orthop Res. 2018. PMID: 28851112 Free PMC article. - The Fragile X mental retardation protein regulates matrix metalloproteinase 9 mRNA at synapses.
Janusz A, Milek J, Perycz M, Pacini L, Bagni C, Kaczmarek L, Dziembowska M. Janusz A, et al. J Neurosci. 2013 Nov 13;33(46):18234-41. doi: 10.1523/JNEUROSCI.2207-13.2013. J Neurosci. 2013. PMID: 24227732 Free PMC article. - A game of tag: MAPS catches up on RNA interactomes.
Carrier MC, Lalaouna D, Massé E. Carrier MC, et al. RNA Biol. 2016 May 3;13(5):473-6. doi: 10.1080/15476286.2016.1156830. Epub 2016 Mar 11. RNA Biol. 2016. PMID: 26967018 Free PMC article. - Simple rules for passive diffusion through the nuclear pore complex.
Timney BL, Raveh B, Mironska R, Trivedi JM, Kim SJ, Russel D, Wente SR, Sali A, Rout MP. Timney BL, et al. J Cell Biol. 2016 Oct 10;215(1):57-76. doi: 10.1083/jcb.201601004. Epub 2016 Oct 3. J Cell Biol. 2016. PMID: 27697925 Free PMC article. - Context-dependent deposition and regulation of mRNAs in P-bodies.
Wang C, Schmich F, Srivatsa S, Weidner J, Beerenwinkel N, Spang A. Wang C, et al. Elife. 2018 Jan 3;7:e29815. doi: 10.7554/eLife.29815. Elife. 2018. PMID: 29297464 Free PMC article.
References
- Amberg DC, Burke D, Strathern JN. Methods in yeast genetics: A Cold Spring Harbor Laboratory course manual. Cold Spring Harbor Laboratory Press; Cold Spring Harbor, New York: 2005. 2005.
- Beach DL, Salmon ED, Bloom K. Localization and anchoring of mRNA in budding yeast. Curr. Biol. 1999;9:569–578. - PubMed
- Bertrand E, Chartrand P, Schaefer M, Shenoy SM, Singer RH, Long RM. Localization of ASH1 mRNA particles in living yeast. Mol. Cell. 1998;2:437–445. - PubMed
LinkOut - more resources
Full Text Sources
Molecular Biology Databases