Structure of the C-terminal domain of Saccharomyces cerevisiae Nup133, a component of the nuclear pore complex - PubMed (original) (raw)

doi: 10.1002/prot.22973. Epub 2011 Mar 1.

Tarun Gheyi, Stacy A Miller, Kevin T Bain, Mark Dickey, Jeffrey B Bonanno, Seung Joong Kim, Jeremy Phillips, Ursula Pieper, Javier Fernandez-Martinez, Josef D Franke, Anne Martel, Hiro Tsuruta, Shane Atwell, Devon A Thompson, J Spencer Emtage, Stephen R Wasserman, Michael P Rout, Andrej Sali, J Michael Sauder, Stephen K Burley

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Structure of the C-terminal domain of Saccharomyces cerevisiae Nup133, a component of the nuclear pore complex

Parthasarathy Sampathkumar et al. Proteins. 2011 May.

Abstract

Nuclear pore complexes (NPCs), responsible for the nucleo-cytoplasmic exchange of proteins and nucleic acids, are dynamic macromolecular assemblies forming an eight-fold symmetric co-axial ring structure. Yeast (Saccharomyces cerevisiae) NPCs are made up of at least 456 polypeptide chains of ~30 distinct sequences. Many of these components (nucleoporins, Nups) share similar structural motifs and form stable subcomplexes. We have determined a high-resolution crystal structure of the C-terminal domain of yeast Nup133 (ScNup133), a component of the heptameric Nup84 subcomplex. Expression tests yielded ScNup133(944-1157) that produced crystals diffracting to 1.9Å resolution.

ScNup133(944-1157) adopts essentially an all α-helical fold, with a short two stranded β-sheet at the C-terminus. The 11 α-helices of ScNup133(944-1157) form a compact fold. In contrast, the previously determined structure of human Nup133(934-1156) bound to a fragment of human Nup107 has its constituent α-helices are arranged in two globular blocks. These differences may reflect structural divergence among homologous nucleoporins.

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Figures

Figure 1

Figure 1

A: Stereo view of a ribbon representation of ScNup133(944-1157). Secondary structure elements were assigned by DSSP. The fold is colored blue to red from the N- to C-termini. B: Comparison of the merged experimental SAXS profile (red) of ScNup133(946-1157) with SAXS profiles computed by IMP-FoXS , (blue) for the complete model of ScNup133(944-1157). Inset shows the SAXS profiles in the Guinier plot. Dmax of radial distribution function, P(r) is 56.25Å). C: The shape of ScNup133(944-1157) derived from the experimental SAXS profile, shown in two orthogonal orientations.

Figure 2

Figure 2

A: Structural superposition of the C-terminal domains of Sc and Hs Nup133. ScNup133(944-1157) is shown as in Fig. 1 (A) and HuNup133(934-1156) is shown in grey.

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