U1-specific protein C needed for efficient complex formation of U1 snRNP with a 5' splice site - PubMed (original) (raw)
. 1990 Jan 5;247(4938):69-72.
doi: 10.1126/science.2136774.
Affiliations
- PMID: 2136774
- DOI: 10.1126/science.2136774
U1-specific protein C needed for efficient complex formation of U1 snRNP with a 5' splice site
V Heinrichs et al. Science. 1990.
Abstract
One of the functions of U1 small nuclear ribonucleoprotein (snRNP) in the splicing reaction of pre-mRNA molecules is the recognition of the 5' splice site. U1 snRNP proteins as well as base-pair interactions between U1 snRNA and the 5' splice site are important for the formation of the snRNP-pre-mRNA complex. To determine which proteins are needed for complex formation, the ability of U1 snRNPs gradually depleted of the U1-specific proteins C, A, and 70k to bind to an RNA molecule containing a 5' splice site sequence was studied in a nitrocellulose filter binding assay. The most significant effect was always observed when protein C was removed, either alone or together with other U1-specific proteins; the binding was reduced by 50 to 60%. Complementation of protein C-deficient U1 snRNPs with purified C protein restored their 5' splice site binding activity. These data suggest that protein C may potentiate the base-pair interaction between U1 RNA and the 5' splice site.
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