Functions of the myosin ATP and actin binding sites are required for C. elegans thick filament assembly - PubMed (original) (raw)
Functions of the myosin ATP and actin binding sites are required for C. elegans thick filament assembly
A Bejsovec et al. Cell. 1990.
Abstract
We have determined the positions and sequences of 31 dominant mutations affecting a C. elegans muscle myosin heavy chain gene. These mutations alter thick filament structure in heterozygotes by interfering with the ability of wild-type myosin to assemble into stable thick filaments. These assembly-disruptive mutations are missense alleles affecting the globular head of myosin. The most strongly dominant alleles alter highly conserved residues of the myosin ATP binding site, indicating that functions of the myosin ATPase are important for thick filament assembly. Other alleles alter the site at which myosin binds actin.
Similar articles
- Myosin heavy-chain mutations that disrupt Caenorhabditis elegans thick filament assembly.
Bejsovec A, Anderson P. Bejsovec A, et al. Genes Dev. 1988 Oct;2(10):1307-17. doi: 10.1101/gad.2.10.1307. Genes Dev. 1988. PMID: 3203908 - Genetic analysis of myosin assembly in Caenorhabditis elegans.
Epstein HF. Epstein HF. Mol Neurobiol. 1990 Spring-Summer;4(1-2):1-25. doi: 10.1007/BF02935583. Mol Neurobiol. 1990. PMID: 2076218 Review. - Molecular and ultrastructural defects in a Drosophila myosin heavy chain mutant: differential effects on muscle function produced by similar thick filament abnormalities.
O'Donnell PT, Bernstein SI. O'Donnell PT, et al. J Cell Biol. 1988 Dec;107(6 Pt 2):2601-12. doi: 10.1083/jcb.107.6.2601. J Cell Biol. 1988. PMID: 2462566 Free PMC article. - Sequence analysis of mutations that affect the synthesis, assembly and enzymatic activity of the unc-54 myosin heavy chain of Caenorhabditis elegans.
Dibb NJ, Brown DM, Karn J, Moerman DG, Bolten SL, Waterston RH. Dibb NJ, et al. J Mol Biol. 1985 Jun 25;183(4):543-51. doi: 10.1016/0022-2836(85)90170-6. J Mol Biol. 1985. PMID: 4020869 - [Myosin head structure].
Levitskiĭ DI. Levitskiĭ DI. Biokhimiia. 1991 Sep;56(9):1539-66. Biokhimiia. 1991. PMID: 1747421 Review. Russian.
Cited by
- Substantial rDNA copy number reductions alter timing of development and produce variable tissue-specific phenotypes in C. elegans.
Morton EA, Hall AN, Cuperus JT, Queitsch C. Morton EA, et al. Genetics. 2023 May 4;224(1):iyad039. doi: 10.1093/genetics/iyad039. Genetics. 2023. PMID: 36919976 Free PMC article. - Mechanical instability generated by Myosin 19 contributes to mitochondria cristae architecture and OXPHOS.
Shi P, Ren X, Meng J, Kang C, Wu Y, Rong Y, Zhao S, Jiang Z, Liang L, He W, Yin Y, Li X, Liu Y, Huang X, Sun Y, Li B, Wu C. Shi P, et al. Nat Commun. 2022 May 13;13(1):2673. doi: 10.1038/s41467-022-30431-3. Nat Commun. 2022. PMID: 35562374 Free PMC article. - Coordination of mitochondrial and cellular dynamics by the actin-based motor Myo19.
Majstrowicz K, Honnert U, Nikolaus P, Schwarz V, Oeding SJ, Hemkemeyer SA, Bähler M. Majstrowicz K, et al. J Cell Sci. 2021 May 15;134(10):jcs255844. doi: 10.1242/jcs.255844. Epub 2021 May 20. J Cell Sci. 2021. PMID: 34013964 Free PMC article. - Local Myo9b RhoGAP activity regulates cell motility.
Hemkemeyer SA, Vollmer V, Schwarz V, Lohmann B, Honnert U, Taha M, Schnittler HJ, Bähler M. Hemkemeyer SA, et al. J Biol Chem. 2021 Jan-Jun;296:100136. doi: 10.1074/jbc.RA120.013623. Epub 2020 Dec 6. J Biol Chem. 2021. PMID: 33268376 Free PMC article. - The R249Q hypertrophic cardiomyopathy myosin mutation decreases contractility in Drosophila by impeding force production.
Bell KM, Kronert WA, Huang A, Bernstein SI, Swank DM. Bell KM, et al. J Physiol. 2019 May;597(9):2403-2420. doi: 10.1113/JP277333. Epub 2019 Apr 4. J Physiol. 2019. PMID: 30950055 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Medical