Centrally injected kisspeptin reduces food intake by increasing meal intervals in mice - PubMed (original) (raw)

Centrally injected kisspeptin reduces food intake by increasing meal intervals in mice

Andreas Stengel et al. Neuroreport. 2011.

Abstract

Kisspeptin is distributed not only in brain areas for regulating reproduction but also in nuclei involved in feeding control. Whether kisspeptin alters food intake is unknown in mice. We examined how kisspeptin-10 influences feeding after intracerebroventricular injection in mice using automated monitoring. Kisspeptin-10 (0.3, 1, and 3 μg/mouse) dose-dependently inhibited the feeding response to an overnight fast by 50, 95, and 90% respectively, during the 2-3 h period postinjection. The 1μg/mouse dose reduced the 4-h cumulative food intake by 28% whereas intraperitoneal injection (10 μg/mouse) did not. The decreased 4-h food intake was due to reduced meal frequency (-45%/4 h), whereas meal size and gastric emptying were not altered. These data suggest that kisspeptin may be a negative central regulator of feeding by increasing satiety.

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Figures

Figure 1

Figure 1

Intracerebroventricular injection of kisspeptin-10 reduces the food intake response to an overnight fast in mice. Animals deprived of food for 17 h were ICV injected with kisspeptin-10 (0.3, 1 or 3 µg/mouse) or vehicle at 9 am and food ingestion was automatically recorded for 24 h and expressed as (a) food intake/1 h periods during the first 4 h, (b) food intake/4 h periods over 24 h and (c) cumulative food intake over 24 h. *P<0.05 and **P<0.01 vs. vehicle.

Figure 2

Figure 2

Intracerebroventricular injection of kisspeptin-10 alters the microstructure of feeding response to an overnight fast in mice. Animals deprived of food for 17 h were injected with kisspeptin-10 (1 µg/mouse, ICV) or vehicle and food intake microstructure was recorded for 24 h. The feeding microstructure parameters encompassing (a) meal frequency, (b) bout frequency, (c) meal size, (d) meal duration, (e) total meal time, (f) time spent in meals, (g) inter-meal interval, (h) eating rate and (i) satiety ratio are displayed for the first 4 h post injection. *P<0.05 vs. vehicle.

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